Comparison of different conditions for DNA extraction in sputum - a pilot study

Comparison of different conditions for DNA extraction in sputum - a pilot study

Abstract Background The analysis of microbiome in respiratory samples is a topic of great interest in chronic respiratory diseases. The method used to prepare sputum samples for microbiome analysis is very heterogeneous. The selection of the most suitable methodology for DNA extraction is fundamenta...

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Bibliographic Details
Main Authors: Martina Oriano, Leonardo Terranova, Antonio Teri, Samantha Sottotetti, Luca Ruggiero, Camilla Tafuro, Paola Marchisio, Andrea Gramegna, Francesco Amati, Fabrizio Nava, Elisa Franceschi, Lisa Cariani, Francesco Blasi, Stefano Aliberti
Format: Article
Language:English
Published: Mattioli 1885 2019-01-01
Series:Multidisciplinary Respiratory Medicine
Subjects:
DNA extraction
Sputum
Microbiota
Microbiome
Sequencing
Online Access:http://link.springer.com/article/10.1186/s40248-018-0166-z

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http://link.springer.com/article/10.1186/s40248-018-0166-z

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