Red Blood Cell Lysis Pretreatment Can Significantly Improve the Yield of Treponema pallidum DNA from Blood

Red Blood Cell Lysis Pretreatment Can Significantly Improve the Yield of Treponema pallidum DNA from Blood

ABSTRACT PCR can be a supplement to Treponema serological testing. However, its sensitivity is not satisfactory for blood sample testing. The aim of this study was to investigate whether pretreatment with red blood cell (RBC) lysis could enhance the yield of Treponema pallidum subsp. pallidum DNA ex...

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Bibliographic Details
Main Authors: Zi-Han Wei, Lin Xie, Yong-Jing Wang, Jiang-Xing Zhuang, Jian-Jun Niu, Li-Li Liu
Format: Article
Language:English
Published: American Society for Microbiology 2023-06-01
Series:Microbiology Spectrum
Subjects:
PCR
sample preparation
blood
blood component
DNA yield
Treponema pallidum
Online Access:https://journals.asm.org/doi/10.1128/spectrum.05198-22

Internet

https://journals.asm.org/doi/10.1128/spectrum.05198-22

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