Simple enzymatic means to neutralize DNA contamination in nucleic acid amplification
Reverse transcription PCR (RT-PCR) is prone to false positives when contaminating DNA molecules are present at the start of a reaction. Contaminants that derive from earlier work using a given primer pair (carryover PCR products) are of particular concern when those primers are used routinely, as in...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-07-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/05391ST02 |