Direct supercritical angle localization microscopy for nanometer 3D superresolution

Direct supercritical angle localization microscopy for nanometer 3D superresolution

Supercritical angle localisation microscopy (SALM) allows the z-positions of single fluorophores to be extracted from the intensity of supercritical angle fluorescence. Here the authors improve the z-resolution of SALM, and report nanometre isotropic localisation precision on DNA origami structures.

Bibliographic Details
Main Authors: Anindita Dasgupta, Joran Deschamps, Ulf Matti, Uwe Hübner, Jan Becker, Sebastian Strauss, Ralf Jungmann, Rainer Heintzmann, Jonas Ries
Format: Article
Language:English
Published: Nature Portfolio 2021-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-021-21333-x

Internet

https://doi.org/10.1038/s41467-021-21333-x

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