Next generation VAMS®–Trypsin immobilization for instant proteolysis in bottom-up protein determination

The purpose of this study was to develop a smart proteolysis sampler based on commercially available VAMS® tips employing Volumetric Absorptive Microsampling. In this proof-of-concept paper we show that covalently bound trypsin to VAMS exhibits sufficient activity for proteomic purposes without alte...

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Bibliographic Details
Main Authors: Léon Reubsaet, Bernd Thiede, Trine Grønhaug Halvorsen
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:Advances in Sample Preparation
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2772582022000249