A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

A simple method for in-house Pfu DNA polymerase purification for high-fidelity PCR amplification

Background and Objectives: Pfu DNA polymerase is an enzyme that exhibits the lowest error rate in the 3´ to 5´ exonuclease (proofreading) activity during DNA synthesis in Polymerase Chain Reactions (PCRs). This study was aimed to express and purify Pfu DNA polymerase in a bacterial expression system...

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Bibliographic Details
Main Authors: Prabu Siva Sankar, Marimuthu Citartan, Aminah Ahmed Siti, Boris V. Skryabin, Timofey S. Rozhdestvensky, Goot Heah Khor, Thean Hock Tang
Format: Article
Language:English
Published: Tehran University of Medical Sciences 2019-05-01
Series:Iranian Journal of Microbiology
Subjects:
Protein expression
Pyrococcus furiosus
DNA polymerase
Polymerase chain reaction
Online Access:https://ijm.tums.ac.ir/index.php/ijm/article/view/1929

Internet

https://ijm.tums.ac.ir/index.php/ijm/article/view/1929

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