Theoretical and Experimental Considerations for a Rapid and High Throughput Measurement of Catalase In Vitro

Theoretical and Experimental Considerations for a Rapid and High Throughput Measurement of Catalase In Vitro

A rapid and high throughput protocol to measure the catalase activity in vitro has been designed. Catalase is an enzyme with unusual kinetic properties because it does not follow the standard Michaelis–Menten model and is inactivated by H<sub>2</sub>O<sub>2</sub>. This makes...

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Bibliographic Details
Main Authors: Ouardia Bendou, Ismael Gutiérrez-Fernández, Emilio L. Marcos-Barbero, Nara Bueno-Ramos, Ana I. González-Hernández, Rosa Morcuende, Juan B. Arellano
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Antioxidants
Subjects:
catalase
hydrogen peroxide
microplate reader
polynomial fitting
suicide substrate
Online Access:https://www.mdpi.com/2076-3921/11/1/21

Internet

https://www.mdpi.com/2076-3921/11/1/21

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