Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells

Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the sa...

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Bibliographic Details
Main Authors: Aida Menéndez-Méndez, Lucía Paniagua-Herranz, Luis A. Olivos-Oré, Rosa Gómez-Villafuertes, Raquel Pérez-Sen, Esmerilda G. Delicado, Antonio R. Artalejo, Felipe Ortega
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006705