Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells
Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the sa...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006705 |
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| author | Aida Menéndez-Méndez Lucía Paniagua-Herranz Luis A. Olivos-Oré Rosa Gómez-Villafuertes Raquel Pérez-Sen Esmerilda G. Delicado Antonio R. Artalejo Felipe Ortega |
| author_facet | Aida Menéndez-Méndez Lucía Paniagua-Herranz Luis A. Olivos-Oré Rosa Gómez-Villafuertes Raquel Pérez-Sen Esmerilda G. Delicado Antonio R. Artalejo Felipe Ortega |
| author_sort | Aida Menéndez-Méndez |
| collection | DOAJ |
| description | Summary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings.For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b). |
| first_indexed | 2024-12-21T23:47:02Z |
| format | Article |
| id | doaj.art-8c1d8c0273374a7580a20f18f218acef |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-21T23:47:02Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-8c1d8c0273374a7580a20f18f218acef2022-12-21T18:46:04ZengElsevierSTAR Protocols2666-16672021-12-0124100964Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cellsAida Menéndez-Méndez0Lucía Paniagua-Herranz1Luis A. Olivos-Oré2Rosa Gómez-Villafuertes3Raquel Pérez-Sen4Esmerilda G. Delicado5Antonio R. Artalejo6Felipe Ortega7Department of Biochemistry and Molecular Biology, Faculty of Veterinary, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; Instituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; Instituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, Spain; Corresponding authorInstituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, Spain; Department of Pharmacology and Toxicology, Faculty of Veterinary, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; Instituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; Instituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; Instituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, SpainInstituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, Spain; Department of Pharmacology and Toxicology, Faculty of Veterinary, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartment of Biochemistry and Molecular Biology, Faculty of Veterinary, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain; Instituto Universitario de Investigación en Neuroquímica (IUIN), 28029 Madrid, Spain; Instituto de Investigación Sanitaria San Carlos (IdISSC), 28040 Madrid, Spain; Corresponding authorSummary: Low-density cell culture of the postnatal cerebellum, combined with live imaging and single-cell tracking, allows the behavior of postnatal cerebellar neural stem cells (NSCs) and their progeny to be monitored. Cultured cerebellar NSCs maintain their neurogenic nature giving rise, in the same relative proportions that exist in vivo, to the neuronal progeny generated by the three postnatal cerebellar neurogenic niches. This protocol describes the identification of the nature of the progeny through both post-imaging immunocytochemistry and patch-clamp recordings.For complete details on the use and execution of this protocol, please refer to Paniagua-Herranz et al. (2020b).http://www.sciencedirect.com/science/article/pii/S2666166721006705Cell BiologyCell cultureSingle CellDevelopmental biologyMicroscopyNeuroscience |
| spellingShingle | Aida Menéndez-Méndez Lucía Paniagua-Herranz Luis A. Olivos-Oré Rosa Gómez-Villafuertes Raquel Pérez-Sen Esmerilda G. Delicado Antonio R. Artalejo Felipe Ortega Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells STAR Protocols Cell Biology Cell culture Single Cell Developmental biology Microscopy Neuroscience |
| title | Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells |
| title_full | Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells |
| title_fullStr | Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells |
| title_full_unstemmed | Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells |
| title_short | Combining low-density cell culture, single-cell tracking, and patch-clamp to monitor the behavior of postnatal murine cerebellar neural stem cells |
| title_sort | combining low density cell culture single cell tracking and patch clamp to monitor the behavior of postnatal murine cerebellar neural stem cells |
| topic | Cell Biology Cell culture Single Cell Developmental biology Microscopy Neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721006705 |
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