Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers.

As an integral membrane protein, purification and characterization of phospho-N- acetylmuramyl- pentapeptide translocase MraY have proven difficult. Low yield and concerns of retaining stability and activity after detergent solubilization have hampered the structure-function analysis. The recently d...

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Bibliographic Details
Main Authors: Yao Liu, Elisabete C C M Moura, Jonas M Dörr, Stefan Scheidelaar, Michal Heger, Maarten R Egmond, J Antoinette Killian, Tamimount Mohammadi, Eefjan Breukink
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC6218056?pdf=render