A novel assay for the introduction of the vinyl ether double bond into plasmalogens using pyrene-labeled substrates

A novel assay for the introduction of the vinyl ether double bond into plasmalogens using pyrene-labeled substrates

Plasmanylethanolamine desaturase (PEDS) (EC 1.14.99.19) introduces the 1-prime double bond into plasmalogens, one of the most abundant phospholipids in the human body. This labile membrane enzyme has not been purified and its coding sequence is unknown. Previous assays for this enzyme used radiolabe...

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Bibliographic Details
Main Authors: Ernst R. Werner, Markus A. Keller, Sabrina Sailer, Daniele Seppi, Georg Golderer, Gabriele Werner-Felmayer, Raphael A. Zoeller, Katrin Watschinger
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:Journal of Lipid Research
Subjects:
ether lipid
plasmanylethanolamine desaturase
phosphatidyl ethanolamine
lyso-phosphatidyl ethanolamine
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520331254

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http://www.sciencedirect.com/science/article/pii/S0022227520331254

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