Comparison of two commercial DNA extraction kits for the analysis of nasopharyngeal bacterial communities

Comparison of two commercial DNA extraction kits for the analysis of nasopharyngeal bacterial communities

Characterization of microbial communities via next-generation sequencing (NGS) requires an extraction ofmicrobial DNA. Methodological differences in DNA extraction protocols may bias results and complicate inter-study comparisons. Here we compare the effect of two commonly used commercial kits (Norg...

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Bibliographic Details
Main Authors: Keith A. Crandall, Robert J. Freishtat, Marcos Pérez-Losada
Format: Article
Language:English
Published: AIMS Press 2016-04-01
Series:AIMS Microbiology
Subjects:
16S rRNA
asthma
DNA extraction
metataxonomics
microbiome
nasopharynx
Online Access:http://www.aimspress.com/microbiology/article/761/fulltext.html

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http://www.aimspress.com/microbiology/article/761/fulltext.html

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