Reference genome and transcriptome informed by the sex chromosome complement of the sample increase ability to detect sex differences in gene expression from RNA-Seq data

Reference genome and transcriptome informed by the sex chromosome complement of the sample increase ability to detect sex differences in gene expression from RNA-Seq data

Abstract Background Human X and Y chromosomes share an evolutionary origin and, as a consequence, sequence similarity. We investigated whether the sequence homology between the X and Y chromosomes affects the alignment of RNA-Seq reads and estimates of differential expression. We tested the effects...

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Bibliographic Details
Main Authors: Kimberly C. Olney, Sarah M. Brotman, Jocelyn P. Andrews, Valeria A. Valverde-Vesling, Melissa A. Wilson
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Biology of Sex Differences
Subjects:
RNA-Seq
Sex chromosomes
Differential expression
Transcriptome
Mapping
Alignment
Online Access:http://link.springer.com/article/10.1186/s13293-020-00312-9

Internet

http://link.springer.com/article/10.1186/s13293-020-00312-9

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