Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films
Ultrasonic-assisted extraction of buckwheat bran flavonoids (BBF) was used. The effects of pH, temperature, light condition and metal ions on the stability of flavonoids were determined. The antioxidant activity of flavonoids in vitro was analyzed by DPPH·, iron reductive force, ABTS+·, superoxide a...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-10-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120140 |
| _version_ | 1797661857782169600 |
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| author | Peipei GUO Lin LI Lina JIN Zhong ZHANG Jing FU |
| author_facet | Peipei GUO Lin LI Lina JIN Zhong ZHANG Jing FU |
| author_sort | Peipei GUO |
| collection | DOAJ |
| description | Ultrasonic-assisted extraction of buckwheat bran flavonoids (BBF) was used. The effects of pH, temperature, light condition and metal ions on the stability of flavonoids were determined. The antioxidant activity of flavonoids in vitro was analyzed by DPPH·, iron reductive force, ABTS+·, superoxide anion and other methods. The buckwheat bran flavone-chitosan blend films (BBF-CS) were prepared using BBF and chitosan (CS) as materials. The effects of adding BBF (0, 5, 10, 15, 20 mL) on the thickness, opacity, chroma, solubility and mechanical properties of the blend films were determined. The results showed that BBF was suitable for storing in a weak acid (pH5~7) environment under normal temperature and avoiding the contact of Ca2+, Fe3+and Cu2+. In vitro antioxidant activity assay revealed that BBF had significant scavenging capacity for DPPH, ABTS+ free radicals and superoxide anion, with the maximum scavenging rate of 92.1% and 87.7%, 53.81%, respectively, and the absorbance value of reducing power for iron ion reached 3.54. Compared with CS film, BBF-CS blend films had higher thickness, opacity and yellowness, while L* value, total color difference, water content, solubility, tensile strength and elongation at break was lower. The method is stable and feasible, and can be used to extract BBF with strong antioxidant activity, will provide a basis for the subsequent development of BBF-CS blend films in food active packaging resources. |
| first_indexed | 2024-03-11T18:51:47Z |
| format | Article |
| id | doaj.art-8c7d19f7646c431a8256dfaa0bcc3d66 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T18:51:47Z |
| publishDate | 2023-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-8c7d19f7646c431a8256dfaa0bcc3d662023-10-11T09:18:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-10-01442026527210.13386/j.issn1002-0306.20221201402022120140-20Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend FilmsPeipei GUO0Lin LI1Lina JIN2Zhong ZHANG3Jing FU4School of Food Science and Engineering, Ningxia University, Yinchuan 750021, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan 750021, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan 750021, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan 750021, ChinaSchool of Food Science and Engineering, Ningxia University, Yinchuan 750021, ChinaUltrasonic-assisted extraction of buckwheat bran flavonoids (BBF) was used. The effects of pH, temperature, light condition and metal ions on the stability of flavonoids were determined. The antioxidant activity of flavonoids in vitro was analyzed by DPPH·, iron reductive force, ABTS+·, superoxide anion and other methods. The buckwheat bran flavone-chitosan blend films (BBF-CS) were prepared using BBF and chitosan (CS) as materials. The effects of adding BBF (0, 5, 10, 15, 20 mL) on the thickness, opacity, chroma, solubility and mechanical properties of the blend films were determined. The results showed that BBF was suitable for storing in a weak acid (pH5~7) environment under normal temperature and avoiding the contact of Ca2+, Fe3+and Cu2+. In vitro antioxidant activity assay revealed that BBF had significant scavenging capacity for DPPH, ABTS+ free radicals and superoxide anion, with the maximum scavenging rate of 92.1% and 87.7%, 53.81%, respectively, and the absorbance value of reducing power for iron ion reached 3.54. Compared with CS film, BBF-CS blend films had higher thickness, opacity and yellowness, while L* value, total color difference, water content, solubility, tensile strength and elongation at break was lower. The method is stable and feasible, and can be used to extract BBF with strong antioxidant activity, will provide a basis for the subsequent development of BBF-CS blend films in food active packaging resources.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120140buckwheat branflavonoidsstabilityantioxidant activityblend films |
| spellingShingle | Peipei GUO Lin LI Lina JIN Zhong ZHANG Jing FU Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films Shipin gongye ke-ji buckwheat bran flavonoids stability antioxidant activity blend films |
| title | Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films |
| title_full | Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films |
| title_fullStr | Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films |
| title_full_unstemmed | Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films |
| title_short | Stability and Antioxidant Activity Analysis of Buckwheat Bran Flavonoids and Preparation of Chitosan Blend Films |
| title_sort | stability and antioxidant activity analysis of buckwheat bran flavonoids and preparation of chitosan blend films |
| topic | buckwheat bran flavonoids stability antioxidant activity blend films |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120140 |
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