ISOLATION AND PURIFICATION OF AVIDIN FROM EGG WHITE BY IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY
Lysozyme and avidin were initially separated from egg white by cation exchange chromatography (Doulite C-464). Avidin was then isolated from lysozyme in pure form by immobilized metal affinity chromatography column (1.5 x 10cm) loaded with copper ions. The column was equilibrated with 0.02M phospha...
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Format: | Article |
Language: | English |
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University of Baghdad, College of Veterinary Medicine
1996-12-01
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Series: | The Iraqi Journal of Veterinary Medicine |
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Online Access: | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1359 |