Solid-state NMR for the characterization of retinal chromophore and Schiff base in TAT rhodopsin embedded in membranes under weakly acidic conditions

Solid-state NMR for the characterization of retinal chromophore and Schiff base in TAT rhodopsin embedded in membranes under weakly acidic conditions

TAT rhodopsin extracted from the marine bacterium SAR11 HIMB114 has a characteristic Thr-Ala-Thr motif and contains both protonated and deprotonated states of Schiff base at physiological pH conditions due to the low pKa. Here, using solid-state NMR spectroscopy, we investigated the 13C and 15N NMR...

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Bibliographic Details
Main Authors: Sui Arikawa, Teppei Sugimoto, Takashi Okitsu, Akimori Wada, Kota Katayama, Hideki Kandori, Izuru Kawamura
Format: Article
Language:English
Published: The Biophysical Society of Japan 2023-03-01
Series:Biophysics and Physicobiology
Subjects:
microbial rhodopsin
solid-state nuclear magnetic resonance
retinal protonated schiff base
hydrogen bond
chemical shift
Online Access:https://doi.org/10.2142/biophysico.bppb-v20.s017

Internet

https://doi.org/10.2142/biophysico.bppb-v20.s017

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