Defective microglial development in the hippocampus of Cx3cr1 deficient mice
Microglial cells participate in brain development and influence neuronal loss and synaptic maturation. Fractalkine is an important neuronal chemokine whose expression increases during development and that can influence microglia function via the fractalkine receptor, CX3CR1. Mice lacking Cx3cr1 show...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-03-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00111/full |
| _version_ | 1818494101666922496 |
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| author | Francesca ePagani Rosa ePaolicelli Rosa ePaolicelli Emanuele eMurana Barbara eCortese Silvia eDi Angelantonio Silvia eDi Angelantonio Emanuele eZurolo Eva eGuiducci Tiago A. eFerreira Stefano eGarofalo Myriam eCatalano Myriam eCatalano Giuseppina eD'Alessandro Alessandra ePorzia Giovanna ePeruzzi Fabrizio eMainiero Cristina eLimatola Cristina eLimatola Cornelius eGross Davide eRagozzino Davide eRagozzino |
| author_facet | Francesca ePagani Rosa ePaolicelli Rosa ePaolicelli Emanuele eMurana Barbara eCortese Silvia eDi Angelantonio Silvia eDi Angelantonio Emanuele eZurolo Eva eGuiducci Tiago A. eFerreira Stefano eGarofalo Myriam eCatalano Myriam eCatalano Giuseppina eD'Alessandro Alessandra ePorzia Giovanna ePeruzzi Fabrizio eMainiero Cristina eLimatola Cristina eLimatola Cornelius eGross Davide eRagozzino Davide eRagozzino |
| author_sort | Francesca ePagani |
| collection | DOAJ |
| description | Microglial cells participate in brain development and influence neuronal loss and synaptic maturation. Fractalkine is an important neuronal chemokine whose expression increases during development and that can influence microglia function via the fractalkine receptor, CX3CR1. Mice lacking Cx3cr1 show a variety of neuronal defects thought to be the result of deficient microglia function. Activation of CX3CR1 is important for the proper migration of microglia to sites of injury and into the brain during development. However, little is known about how fractalkine modulates microglial properties during development. Here we examined microglial morphology, response to ATP, and K+ current properties in acute brain slices from Cx3cr1 knockout mice across postnatal hippocampal development. We found that fractalkine signaling is necessary for the development of several morphological and physiological features of microglia. Specifically, we found that the occurrence of an outward rectifying K+ current, typical of activated microglia, that peaked during the second and third postnatal week, was reduced in Cx3cr1 knockout mice. Fractalkine signaling also influenced microglial morphology and ability to extend processes in response to ATP following its focal application to the slice. Our results reveal the developmental profile of several morphological and physiological properties of microglia and demonstrate that these processes are modulated by fractalkine signaling. |
| first_indexed | 2024-12-10T18:02:17Z |
| format | Article |
| id | doaj.art-8cb62bfc85f44604bac75c98f5f020fe |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-10T18:02:17Z |
| publishDate | 2015-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-8cb62bfc85f44604bac75c98f5f020fe2022-12-22T01:38:44ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022015-03-01910.3389/fncel.2015.00111133241Defective microglial development in the hippocampus of Cx3cr1 deficient miceFrancesca ePagani0Rosa ePaolicelli1Rosa ePaolicelli2Emanuele eMurana3Barbara eCortese4Silvia eDi Angelantonio5Silvia eDi Angelantonio6Emanuele eZurolo7Eva eGuiducci8Tiago A. eFerreira9Stefano eGarofalo10Myriam eCatalano11Myriam eCatalano12Giuseppina eD'Alessandro13Alessandra ePorzia14Giovanna ePeruzzi15Fabrizio eMainiero16Cristina eLimatola17Cristina eLimatola18Cornelius eGross19Davide eRagozzino20Davide eRagozzino21Istituto Italiano di TecnologiaUniversity of ZürichEuropean Molecular Biology LaboratoryIstituto Pasteur-Fondazione Cenci BolognettiSapienza University of RomeIstituto Italiano di TecnologiaIstituto Pasteur-Fondazione Cenci BolognettiUniversity of AmsterdamEuropean Molecular Biology LaboratoryEuropean Molecular Biology LaboratoryIstituto Pasteur-Fondazione Cenci BolognettiIstituto Pasteur-Fondazione Cenci BolognettiIRCCS NeuromedIstituto Pasteur-Fondazione Cenci BolognettiIstituto Pasteur-Fondazione Cenci BolognettiIstituto Italiano di TecnologiaSapienza University of RomeIstituto Pasteur-Fondazione Cenci BolognettiIRCCS NeuromedEuropean Molecular Biology LaboratoryEuropean Molecular Biology LaboratoryIRCCS NeuromedMicroglial cells participate in brain development and influence neuronal loss and synaptic maturation. Fractalkine is an important neuronal chemokine whose expression increases during development and that can influence microglia function via the fractalkine receptor, CX3CR1. Mice lacking Cx3cr1 show a variety of neuronal defects thought to be the result of deficient microglia function. Activation of CX3CR1 is important for the proper migration of microglia to sites of injury and into the brain during development. However, little is known about how fractalkine modulates microglial properties during development. Here we examined microglial morphology, response to ATP, and K+ current properties in acute brain slices from Cx3cr1 knockout mice across postnatal hippocampal development. We found that fractalkine signaling is necessary for the development of several morphological and physiological features of microglia. Specifically, we found that the occurrence of an outward rectifying K+ current, typical of activated microglia, that peaked during the second and third postnatal week, was reduced in Cx3cr1 knockout mice. Fractalkine signaling also influenced microglial morphology and ability to extend processes in response to ATP following its focal application to the slice. Our results reveal the developmental profile of several morphological and physiological properties of microglia and demonstrate that these processes are modulated by fractalkine signaling.http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00111/fullMicrogliadevelopmentrearrangementFractalkineCX3CR1potassium currents |
| spellingShingle | Francesca ePagani Rosa ePaolicelli Rosa ePaolicelli Emanuele eMurana Barbara eCortese Silvia eDi Angelantonio Silvia eDi Angelantonio Emanuele eZurolo Eva eGuiducci Tiago A. eFerreira Stefano eGarofalo Myriam eCatalano Myriam eCatalano Giuseppina eD'Alessandro Alessandra ePorzia Giovanna ePeruzzi Fabrizio eMainiero Cristina eLimatola Cristina eLimatola Cornelius eGross Davide eRagozzino Davide eRagozzino Defective microglial development in the hippocampus of Cx3cr1 deficient mice Frontiers in Cellular Neuroscience Microglia development rearrangement Fractalkine CX3CR1 potassium currents |
| title | Defective microglial development in the hippocampus of Cx3cr1 deficient mice |
| title_full | Defective microglial development in the hippocampus of Cx3cr1 deficient mice |
| title_fullStr | Defective microglial development in the hippocampus of Cx3cr1 deficient mice |
| title_full_unstemmed | Defective microglial development in the hippocampus of Cx3cr1 deficient mice |
| title_short | Defective microglial development in the hippocampus of Cx3cr1 deficient mice |
| title_sort | defective microglial development in the hippocampus of cx3cr1 deficient mice |
| topic | Microglia development rearrangement Fractalkine CX3CR1 potassium currents |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2015.00111/full |
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