A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity

A kinetic model predicts SpCas9 activity, improves off-target classification, and reveals the physical basis of targeting fidelity

Cas9 off-target sites can be predicted by many bioinformatics tools. Here the authors present low complexity mechanistic model that characterizes SpCas9 kinetics in free-energy terms, allowing quantitative prediction of off-target activity in bulk-biochemistry, single molecule, and whole-genome prof...

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Bibliographic Details
Main Authors: Behrouz Eslami-Mossallam, Misha Klein, Constantijn V. D. Smagt, Koen V. D. Sanden, Stephen K. Jones, John A. Hawkins, Ilya J. Finkelstein, Martin Depken
Format: Article
Language:English
Published: Nature Portfolio 2022-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-022-28994-2

Internet

https://doi.org/10.1038/s41467-022-28994-2

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