An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids

Abstract Aminoacyl-tRNA synthetase (aaRS) is a core component for genetic code expansion (GCE), a powerful technique that enables the incorporation of noncanonical amino acids (ncAAs) into a protein. The aaRS with polyspecificity can be exploited in incorporating additional ncAAs into a protein with...

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Bibliographic Details
Main Authors: Ke Liu, Ling Jiang, Shuang Ma, Zhongdi Song, Lun Wang, Qunfeng Zhang, Renhao Xu, Lirong Yang, Jianping Wu, Haoran Yu
Format: Article
Language:English
Published: SpringerOpen 2023-12-01
Series:Bioresources and Bioprocessing
Subjects:
Pyrrolysyl-tRNA synthetase
Genetic code expansion
Noncanonical amino acid
Protein main-chain modification
PedH
Online Access:https://doi.org/10.1186/s40643-023-00712-w

Internet

https://doi.org/10.1186/s40643-023-00712-w

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