CRISPR-Cas9-mediated induction of large chromosomal inversions in human bronchial epithelial cells

CRISPR-Cas9-mediated induction of large chromosomal inversions in human bronchial epithelial cells

Summary: The in vitro recapitulation of chromosomal rearrangements is a necessary tool for understanding malignancy at the molecular level. Here, we describe the targeted induction of a large chromosomal inversion (>3.7 Mbp) through CRISPR-Cas9-mediated genome editing. As inversions occur at low...

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Bibliographic Details
Main Authors: Andriani Angelopoulou, Angelos Papaspyropoulos, Argyris Papantonis, Vassilis G. Gorgoulis
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
Single Cell
Flow Cytometry/Mass Cytometry
Cancer
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672200137X

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http://www.sciencedirect.com/science/article/pii/S266616672200137X

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