Inhibition of Mycelial Growth by Methotrexate in Neurospora crassa Wild Type and Mutants Deficient in Folylpolyglutamate Synthase
In mammalian cells, folylpolyglutamate synthase (FPGS) catalyzes the polyglutamylation of methotrexate (MTX), a reaction that significantly enhances the cellular retention and cytotoxicity of this antifolate. In contrast, MTX is a poor substrate for the cytosolic FPGS of Neurospora crassa. The prese...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
1998-11-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pteridines.1998.9.4.181 |