CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

CRISPR/Cas9-based depletion of 16S ribosomal RNA improves library complexity of single-cell RNA-sequencing in planarians

Abstract Background Single-cell RNA-sequencing (scRNA-seq) relies on PCR amplification to retrieve information from vanishingly small amounts of starting material. To selectively enrich mRNA from abundant non-polyadenylated transcripts, poly(A) selection is a key step during library preparation. How...

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Bibliographic Details
Main Authors: Kuang-Tse Wang, Carolyn E. Adler
Format: Article
Language:English
Published: BMC 2023-10-01
Series:BMC Genomics
Subjects:
scRNA-seq
Ribodepletion
CRISPR/Cas9
DASH
Planarians
Online Access:https://doi.org/10.1186/s12864-023-09724-4

Internet

https://doi.org/10.1186/s12864-023-09724-4

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