Glyoxal Fixation Is Optimal for Immunostaining of Brain Vessels, Pericytes and Blood-Brain Barrier Proteins

Glyoxal Fixation Is Optimal for Immunostaining of Brain Vessels, Pericytes and Blood-Brain Barrier Proteins

Brain vascular staining is very important for understanding cerebrovascular pathologies. 4% paraformaldehyde is considered the gold standard fixation technique for immunohistochemistry and it revolutionized the examination of proteins in fixed tissues. However, this fixation technique produces incon...

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Bibliographic Details
Main Authors: Sithara Thomas, Jayanarayanan Sadanandan, Spiros L. Blackburn, Devin W. McBride, Ari Dienel, Sungha Hong, Hussein A. Zeineddine, Peeyush Kumar Thankamani
Format: Article
Language:English
Published: MDPI AG 2022-07-01
Series:International Journal of Molecular Sciences
Subjects:
glyoxal
paraformaldehyde
vascular staining
blood-brain barrier
tight junction
pericytes
Online Access:https://www.mdpi.com/1422-0067/23/14/7776

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https://www.mdpi.com/1422-0067/23/14/7776

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