PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells

PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells

Abstract Background Base editing via CRISPR-Cas9 has garnered attention as a method for correcting disease-specific mutations without causing double-strand breaks, thereby avoiding large deletions and translocations in the host chromosome. However, its reliance on the protospacer adjacent motif (PAM...

Full description

Bibliographic Details
Main Authors: Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki, Tsukasa Ohmori
Format: Article
Language:English
Published: Nature Portfolio 2023-04-01
Series:Communications Medicine
Online Access:https://doi.org/10.1038/s43856-023-00286-w

Internet

https://doi.org/10.1038/s43856-023-00286-w

Similar Items