Protocol for multiplex fluorescent immunohistochemistry in free-floating rodent brain tissues

Summary: Identifying multiple proteins within the same tissue allows for assessing protein colocalization, is cost effective, and maximizes efficiency. Here, we describe a protocol for multiplex immunolabeling of proteins in free-floating rodent brain sections. As opposed to slide-mounted immunohist...

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Bibliographic Details
Main Authors: Aubrey M. Kelly, Brandon A. Fricker, Kelly J. Wallace
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722005524