SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans
Introduction: The high prevalence of neurodegenerative diseases in our population and the lack of effective treatments encourage the search for new therapeutic targets for these pathologies. We have recently described that submaximal inhibition of the Sarco-Endoplasmic Reticulum Ca2+ ATPase (SERCA),...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2023.1182428/full |
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| author | Silvia Romero-Sanz Elena Caldero-Escudero Pilar Álvarez-Illera Jaime Santo-Domingo Rosalba I. Fonteriz Mayte Montero Javier Álvarez |
| author_facet | Silvia Romero-Sanz Elena Caldero-Escudero Pilar Álvarez-Illera Jaime Santo-Domingo Rosalba I. Fonteriz Mayte Montero Javier Álvarez |
| author_sort | Silvia Romero-Sanz |
| collection | DOAJ |
| description | Introduction: The high prevalence of neurodegenerative diseases in our population and the lack of effective treatments encourage the search for new therapeutic targets for these pathologies. We have recently described that submaximal inhibition of the Sarco-Endoplasmic Reticulum Ca2+ ATPase (SERCA), the main responsible for ER calcium storage, is able to increase lifespan in Caenorhabditis elegans worms by mechanisms involving mitochondrial metabolism and nutrient-sensitive pathways.Methods: We have studied here the effects of submaximal SERCA inhibition in a chemical model of Parkinson’s disease (PD) induced in C. elegans worms by treatment with the mitochondrial complex I inhibitor rotenone. For specific SERCA inhibition, we treated worms with RNAi against sca-1, the sole orthologue of SERCA in C. elegans.Results and Discussion: Our results show that rotenone produces alterations in worms that include decreased lifespan, smaller size, reduced fertility, decreased motility, defecation and pumping rate, increased mitochondrial ROS production, reduced mitochondrial membrane potential and oxygen consumption rate, altered mitochondrial structure, and altered ethanol preference in behavioral studies. Most of these alterations were either fully or partially reversed in worms treated with sca-1 RNAi, suggesting that SERCA inhibition could be a novel pharmacological target in the prevention or treatment of neurodegeneration. |
| first_indexed | 2024-03-13T10:10:40Z |
| format | Article |
| id | doaj.art-8d9690a792c94c79bf35a50d0351af3c |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-03-13T10:10:40Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-8d9690a792c94c79bf35a50d0351af3c2023-05-22T04:24:40ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122023-05-011410.3389/fphar.2023.11824281182428SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegansSilvia Romero-SanzElena Caldero-EscuderoPilar Álvarez-IlleraJaime Santo-DomingoRosalba I. FonterizMayte MonteroJavier ÁlvarezIntroduction: The high prevalence of neurodegenerative diseases in our population and the lack of effective treatments encourage the search for new therapeutic targets for these pathologies. We have recently described that submaximal inhibition of the Sarco-Endoplasmic Reticulum Ca2+ ATPase (SERCA), the main responsible for ER calcium storage, is able to increase lifespan in Caenorhabditis elegans worms by mechanisms involving mitochondrial metabolism and nutrient-sensitive pathways.Methods: We have studied here the effects of submaximal SERCA inhibition in a chemical model of Parkinson’s disease (PD) induced in C. elegans worms by treatment with the mitochondrial complex I inhibitor rotenone. For specific SERCA inhibition, we treated worms with RNAi against sca-1, the sole orthologue of SERCA in C. elegans.Results and Discussion: Our results show that rotenone produces alterations in worms that include decreased lifespan, smaller size, reduced fertility, decreased motility, defecation and pumping rate, increased mitochondrial ROS production, reduced mitochondrial membrane potential and oxygen consumption rate, altered mitochondrial structure, and altered ethanol preference in behavioral studies. Most of these alterations were either fully or partially reversed in worms treated with sca-1 RNAi, suggesting that SERCA inhibition could be a novel pharmacological target in the prevention or treatment of neurodegeneration.https://www.frontiersin.org/articles/10.3389/fphar.2023.1182428/fullC. elegansrotenoneParkinson’s diseaseSERCAlifespanendoplasmic reticulum |
| spellingShingle | Silvia Romero-Sanz Elena Caldero-Escudero Pilar Álvarez-Illera Jaime Santo-Domingo Rosalba I. Fonteriz Mayte Montero Javier Álvarez SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans Frontiers in Pharmacology C. elegans rotenone Parkinson’s disease SERCA lifespan endoplasmic reticulum |
| title | SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans |
| title_full | SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans |
| title_fullStr | SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans |
| title_full_unstemmed | SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans |
| title_short | SERCA inhibition improves lifespan and healthspan in a chemical model of Parkinson disease in Caenorhabditis elegans |
| title_sort | serca inhibition improves lifespan and healthspan in a chemical model of parkinson disease in caenorhabditis elegans |
| topic | C. elegans rotenone Parkinson’s disease SERCA lifespan endoplasmic reticulum |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2023.1182428/full |
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