Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile
Abstract Hyperglycemia leads to microvascular lesions in various tissues. In diabetic nephropathy—DN, alterations in usual markers reflect an already installed disease. The study of new biomarkers for the early detection of diabetic complications can bring new prevention perspectives. Rats were divi...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41061-0 |
| _version_ | 1827634404130816000 |
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| author | Joyce Regina Santos Raimundo Beatriz da Costa Aguiar Alves Jéssica Freitas Araujo Encinas Andressa Moreira Siqueira Katharyna Cardoso de Gois Matheus Moreira Perez Giuliana Petri José Francisco Ramos dos Santos Fernando Luiz Affonso Fonseca Glaucia Luciano da Veiga |
| author_facet | Joyce Regina Santos Raimundo Beatriz da Costa Aguiar Alves Jéssica Freitas Araujo Encinas Andressa Moreira Siqueira Katharyna Cardoso de Gois Matheus Moreira Perez Giuliana Petri José Francisco Ramos dos Santos Fernando Luiz Affonso Fonseca Glaucia Luciano da Veiga |
| author_sort | Joyce Regina Santos Raimundo |
| collection | DOAJ |
| description | Abstract Hyperglycemia leads to microvascular lesions in various tissues. In diabetic nephropathy—DN, alterations in usual markers reflect an already installed disease. The study of new biomarkers for the early detection of diabetic complications can bring new prevention perspectives. Rats were divided into diabetic adult—DMA—or elderly—DME and control sham adult—CSA—or control sham elderly—CSE. Blood and urine samples were collected for biochemical analysis. Bulbar region, cardiac, hepatic and renal tissues were collected for target gene expression studies. As result, DMA showed decreased TNFR1, MCT1 and CD147 expression in the bulbar region, TNFR1 in the heart, VEGFA and CD147 in the kidney and TNFR1 in blood. Positive correlations were found between TNFR1 and MCT1 in the bulbar region and HbA1c and plasma creatinine, respectively. DME showed positive correlation in the bulbar region between TNFR1 and glycemia, in addition to negative correlations between CD147 in the heart versus glycemia and urea. We concluded that the initial hyperglycemic stimulus already promotes changes in the expression of genes involved in the inflammatory and metabolic pathways, and aging alters this profile. These changes prior to the onset of diseases such as DN, show that they have potential for early biomarkers studies. |
| first_indexed | 2024-03-09T15:12:53Z |
| format | Article |
| id | doaj.art-8db52a8c59bd4a269589c90ad3c71036 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:12:53Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-8db52a8c59bd4a269589c90ad3c710362023-11-26T13:15:34ZengNature PortfolioScientific Reports2045-23222023-10-0113111310.1038/s41598-023-41061-0Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profileJoyce Regina Santos Raimundo0Beatriz da Costa Aguiar Alves1Jéssica Freitas Araujo Encinas2Andressa Moreira Siqueira3Katharyna Cardoso de Gois4Matheus Moreira Perez5Giuliana Petri6José Francisco Ramos dos Santos7Fernando Luiz Affonso Fonseca8Glaucia Luciano da Veiga9Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Vivarium and Animal Experimentation Laboratory-Faculdade de Medicina Do ABC (FMABC)Vivarium and Animal Experimentation Laboratory-Faculdade de Medicina Do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Laboratório de Análises Clínicas do Centro Universitário–Faculdade de Medicina do ABC (FMABC)Abstract Hyperglycemia leads to microvascular lesions in various tissues. In diabetic nephropathy—DN, alterations in usual markers reflect an already installed disease. The study of new biomarkers for the early detection of diabetic complications can bring new prevention perspectives. Rats were divided into diabetic adult—DMA—or elderly—DME and control sham adult—CSA—or control sham elderly—CSE. Blood and urine samples were collected for biochemical analysis. Bulbar region, cardiac, hepatic and renal tissues were collected for target gene expression studies. As result, DMA showed decreased TNFR1, MCT1 and CD147 expression in the bulbar region, TNFR1 in the heart, VEGFA and CD147 in the kidney and TNFR1 in blood. Positive correlations were found between TNFR1 and MCT1 in the bulbar region and HbA1c and plasma creatinine, respectively. DME showed positive correlation in the bulbar region between TNFR1 and glycemia, in addition to negative correlations between CD147 in the heart versus glycemia and urea. We concluded that the initial hyperglycemic stimulus already promotes changes in the expression of genes involved in the inflammatory and metabolic pathways, and aging alters this profile. These changes prior to the onset of diseases such as DN, show that they have potential for early biomarkers studies.https://doi.org/10.1038/s41598-023-41061-0 |
| spellingShingle | Joyce Regina Santos Raimundo Beatriz da Costa Aguiar Alves Jéssica Freitas Araujo Encinas Andressa Moreira Siqueira Katharyna Cardoso de Gois Matheus Moreira Perez Giuliana Petri José Francisco Ramos dos Santos Fernando Luiz Affonso Fonseca Glaucia Luciano da Veiga Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile Scientific Reports |
| title | Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile |
| title_full | Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile |
| title_fullStr | Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile |
| title_full_unstemmed | Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile |
| title_short | Expression of TNFR1, VEGFA, CD147 and MCT1 as early biomarkers of diabetes complications and the impact of aging on this profile |
| title_sort | expression of tnfr1 vegfa cd147 and mct1 as early biomarkers of diabetes complications and the impact of aging on this profile |
| url | https://doi.org/10.1038/s41598-023-41061-0 |
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