Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells.
BACKGROUND: Matricellular proteins, including periostin, modulate cell-matrix interactions and cell functions by acting outside of cells. METHODS AND FINDINGS: In this study, however, we reported that periostin physically associates with the Notch1 precursor at its EGF repeats in the inside of cells...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2923609?pdf=render |
| _version_ | 1818391056184508416 |
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| author | Hideyuki Tanabe Issei Takayama Takashi Nishiyama Masashi Shimazaki Isao Kii Minqi Li Norio Amizuka Ken-ichi Katsube Akira Kudo |
| author_facet | Hideyuki Tanabe Issei Takayama Takashi Nishiyama Masashi Shimazaki Isao Kii Minqi Li Norio Amizuka Ken-ichi Katsube Akira Kudo |
| author_sort | Hideyuki Tanabe |
| collection | DOAJ |
| description | BACKGROUND: Matricellular proteins, including periostin, modulate cell-matrix interactions and cell functions by acting outside of cells. METHODS AND FINDINGS: In this study, however, we reported that periostin physically associates with the Notch1 precursor at its EGF repeats in the inside of cells. Moreover, by using the periodontal ligament of molar from periostin-deficient adult mice (Pn-/- molar PDL), which is a constitutively mechanically stressed tissue, we found that periostin maintained the site-1 cleaved 120-kDa transmembrane domain of Notch1 (N1) level without regulating Notch1 mRNA expression. N1 maintenance in vitro was also observed under such a stress condition as heat and H(2)O(2) treatment in periostin overexpressed cells. Furthermore, we found that the expression of a downstream effector of Notch signaling, Bcl-xL was decreased in the Pn-/- molar PDL, and in the molar movement, cell death was enhanced in the pressure side of Pn-/- molar PDL. CONCLUSION: These results suggest the possibility that periostin inhibits cell death through up-regulation of Bcl-xL expression by maintaining the Notch1 protein level under the stress condition, which is caused by its physical association with the Notch1 precursor. |
| first_indexed | 2024-12-14T05:07:27Z |
| format | Article |
| id | doaj.art-8db963c47d0a4a849b9f8f67cb7a3cff |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T05:07:27Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-8db963c47d0a4a849b9f8f67cb7a3cff2022-12-21T23:16:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-0158e1223410.1371/journal.pone.0012234Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells.Hideyuki TanabeIssei TakayamaTakashi NishiyamaMasashi ShimazakiIsao KiiMinqi LiNorio AmizukaKen-ichi KatsubeAkira KudoBACKGROUND: Matricellular proteins, including periostin, modulate cell-matrix interactions and cell functions by acting outside of cells. METHODS AND FINDINGS: In this study, however, we reported that periostin physically associates with the Notch1 precursor at its EGF repeats in the inside of cells. Moreover, by using the periodontal ligament of molar from periostin-deficient adult mice (Pn-/- molar PDL), which is a constitutively mechanically stressed tissue, we found that periostin maintained the site-1 cleaved 120-kDa transmembrane domain of Notch1 (N1) level without regulating Notch1 mRNA expression. N1 maintenance in vitro was also observed under such a stress condition as heat and H(2)O(2) treatment in periostin overexpressed cells. Furthermore, we found that the expression of a downstream effector of Notch signaling, Bcl-xL was decreased in the Pn-/- molar PDL, and in the molar movement, cell death was enhanced in the pressure side of Pn-/- molar PDL. CONCLUSION: These results suggest the possibility that periostin inhibits cell death through up-regulation of Bcl-xL expression by maintaining the Notch1 protein level under the stress condition, which is caused by its physical association with the Notch1 precursor.http://europepmc.org/articles/PMC2923609?pdf=render |
| spellingShingle | Hideyuki Tanabe Issei Takayama Takashi Nishiyama Masashi Shimazaki Isao Kii Minqi Li Norio Amizuka Ken-ichi Katsube Akira Kudo Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. PLoS ONE |
| title | Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. |
| title_full | Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. |
| title_fullStr | Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. |
| title_full_unstemmed | Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. |
| title_short | Periostin associates with Notch1 precursor to maintain Notch1 expression under a stress condition in mouse cells. |
| title_sort | periostin associates with notch1 precursor to maintain notch1 expression under a stress condition in mouse cells |
| url | http://europepmc.org/articles/PMC2923609?pdf=render |
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