Are salty liquid food flavorings in vitro antitumor substances?
ABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419&lng=en&tlng=en |
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| author | FRANCISCO R.S. CARVALHO ANTONIO G. MOURA GARDENIA F. RODRIGUES NARCIA M.F. NUNES DAISY J.B. LIMA CLAUDIA PESSOA MARCILIA P. COSTA PAULO M.P. FERREIRA ANA PAULA PERON |
| author_facet | FRANCISCO R.S. CARVALHO ANTONIO G. MOURA GARDENIA F. RODRIGUES NARCIA M.F. NUNES DAISY J.B. LIMA CLAUDIA PESSOA MARCILIA P. COSTA PAULO M.P. FERREIRA ANA PAULA PERON |
| author_sort | FRANCISCO R.S. CARVALHO |
| collection | DOAJ |
| description | ABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells. |
| first_indexed | 2024-04-12T23:21:32Z |
| format | Article |
| id | doaj.art-8dbd29f2d18a4e52a30c324898243aac |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-12T23:21:32Z |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-8dbd29f2d18a4e52a30c324898243aac2022-12-22T03:12:31ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26908831419143010.1590/0001-3765201620150553S0001-37652016000401419Are salty liquid food flavorings in vitro antitumor substances?FRANCISCO R.S. CARVALHOANTONIO G. MOURAGARDENIA F. RODRIGUESNARCIA M.F. NUNESDAISY J.B. LIMACLAUDIA PESSOAMARCILIA P. COSTAPAULO M.P. FERREIRAANA PAULA PERONABSTRACT The objective of this study was to evaluate the antiproliferative, cytotoxic and genotoxic potential of salty liquid synthetic flavorings of Butter, Cheddar Cheese and Onion. The antiproliferative potential (2.9-1500 µg/mL) was assessed by MTT assay after 72h using the human tumor lines SF-295 (glioblastoma), OVCAR-8 (ovarian), HCT-116 (colon) and HL-60 (promyelocytic leukemia) and primary cultures of murine Sarcoma 180 (S180) and peripheral blood mononuclear cells (PBMC). Allium cepa bulbs were exposed to growing respective doses (1 mL and 2 mL). Only Butter and Cheddar flavorings revealed cytotoxic activity on cancer cells, with IC50 values ranging from 125.4 µg/mL (Cheddar - HCT-116) to 402.6 µg/mL (Butter - OVCAR-8). Butter flavoring was the most cytotoxic on PBMC (136.3 µg/mL) and increased cell division rate in relation to the mitotic index but did not cause cellular aberrations. Onion and Cheddar flavorings reduced the mitotic index after 24h and 48h exposure, but only Onion flavoring resulted in cellular aberrations and mitotic spindle abnormalities, such as anaphase and telophase bridges, micronucleated cells, conchicine-metaphases and amplifications. So, Butter, Onion and/or Cheddar flavorings caused significant changes in the division of meristematic cells of A. cepa and presented cytotoxic action even on decontrolled proliferating human tumor cells.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419&lng=en&tlng=enfood additivesAllium cepaantiproliferative actionneoplasic cellstoxicity |
| spellingShingle | FRANCISCO R.S. CARVALHO ANTONIO G. MOURA GARDENIA F. RODRIGUES NARCIA M.F. NUNES DAISY J.B. LIMA CLAUDIA PESSOA MARCILIA P. COSTA PAULO M.P. FERREIRA ANA PAULA PERON Are salty liquid food flavorings in vitro antitumor substances? Anais da Academia Brasileira de Ciências food additives Allium cepa antiproliferative action neoplasic cells toxicity |
| title | Are salty liquid food flavorings in vitro antitumor substances? |
| title_full | Are salty liquid food flavorings in vitro antitumor substances? |
| title_fullStr | Are salty liquid food flavorings in vitro antitumor substances? |
| title_full_unstemmed | Are salty liquid food flavorings in vitro antitumor substances? |
| title_short | Are salty liquid food flavorings in vitro antitumor substances? |
| title_sort | are salty liquid food flavorings in vitro antitumor substances |
| topic | food additives Allium cepa antiproliferative action neoplasic cells toxicity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000401419&lng=en&tlng=en |
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