A novel fluorescence sensor for milk clotting enzyme chymosin using peptide as substrate and covalent organic framework nanosheet as fluorescence quencher

A novel fluorescence sensor for milk clotting enzyme chymosin using peptide as substrate and covalent organic framework nanosheet as fluorescence quencher

Chymosin is one of the critical enzymes in cheese making. Herein, we proposed a novel fluorometric assay for chymosin determination. Firstly, covalent organic frameworks (COF) were synthesized and exfoliated to 2-dimensional COF nanosheets (COF NS) by ultrasound treatment. Gold nanoparticles (Au NPs...

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Bibliographic Details
Main Authors: Xiaokang Lu, Tianle Qi, Linjiang Guo, Lin Xiao, Hanbin Xu, Guobao Ning, Hui Zhao, Canpeng Li
Format: Article
Language:English
Published: Tsinghua University Press 2024-11-01
Series:Food Science and Human Wellness
Subjects:
covalent organic framework
fluorescence sensor
chymosin
fluorescence resonance energy transfer
Online Access:https://www.sciopen.com/article/10.26599/FSHW.2023.9250042

Internet

https://www.sciopen.com/article/10.26599/FSHW.2023.9250042

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