Development of Nested PCR for SARS-CoV-2 Detection and Its Application for Diagnosis of Active Infection in Cats

SARS-CoV-2 emerged in 2019 and found diagnostic laboratories unprepared worldwide. To meet the need for timely and accurate virus detection, laboratories used rapid Ag tests and PCR kits based on costly multi-channel real-time techniques. This study aimed to develop a conventional nested PCR based o...

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Bibliographic Details
Main Authors: Ivo Sirakov, Ralitsa Popova-Ilinkina, Dobrinka Ivanova, Nikolina Rusenova, Hristiyan Mladenov, Kalina Mihova, Ivan Mitov
Format: Article
Language:English
Published: MDPI AG 2022-06-01
Series:Veterinary Sciences
Subjects:
Online Access:https://www.mdpi.com/2306-7381/9/6/272