Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone
The objective of this work was to examine the effect of controlled doses of O<sub>3</sub> (0, 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min, and 5 µL L<sup>−1</sup> of O<sub>3</sub> for 720 min) on the quality and phytochemical content of...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/15/2195 |
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| author | Arturo Duarte-Sierra Charles F. Forney Minty Thomas Paul Angers Joseph Arul |
| author_facet | Arturo Duarte-Sierra Charles F. Forney Minty Thomas Paul Angers Joseph Arul |
| author_sort | Arturo Duarte-Sierra |
| collection | DOAJ |
| description | The objective of this work was to examine the effect of controlled doses of O<sub>3</sub> (0, 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min, and 5 µL L<sup>−1</sup> of O<sub>3</sub> for 720 min) on the quality and phytochemical content of broccoli florets during postharvest storage. The optimal dose was found at 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min, from the color retention of broccoli florets exposed to the gas treatment. Overall, the antioxidant capacity of the florets was significantly affected by both doses of O<sub>3</sub> compared to the non-exposed florets. The profile of glucosinolates was determined for up to 14 days in broccoli florets stored at 4 °C by LC-MS. The amount of total glucobrassicins and total hydroxy-cinnamates in florets significantly (<i>p</i> ≤ 0.05) improved by the application of 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min compared to non-treated florets. The up-regulation of genes of the tryptophan-derived glucosinolate pathway was observed immediately after both treatments. The gene expression of CYP79A2 and CYP79B3 in broccoli was significantly higher in broccoli florets exposed to 5 µL L<sup>−1</sup> of O<sub>3</sub> for 720 min compared to non-exposed florets. Although enhancement of secondary metabolites can be achieved by the fumigation of broccoli florets with low doses of ozone, quality parameters, particularly weight loss, can be compromised. |
| first_indexed | 2024-03-09T12:38:01Z |
| format | Article |
| id | doaj.art-8dd156342ed848cda2b50cb7a11d04dd |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T12:38:01Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-8dd156342ed848cda2b50cb7a11d04dd2023-11-30T22:22:06ZengMDPI AGFoods2304-81582022-07-011115219510.3390/foods11152195Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of OzoneArturo Duarte-Sierra0Charles F. Forney1Minty Thomas2Paul Angers3Joseph Arul4Food Science Department, Laval University, Quebec, QC G1V 0A6, CanadaKentville Research and Development Centre, Agriculture and Agri-Food Canada, 32 Main Street, Kentville, NS B4N 1J5, CanadaFood Science Department, Laval University, Quebec, QC G1V 0A6, CanadaFood Science Department, Laval University, Quebec, QC G1V 0A6, CanadaFood Science Department, Laval University, Quebec, QC G1V 0A6, CanadaThe objective of this work was to examine the effect of controlled doses of O<sub>3</sub> (0, 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min, and 5 µL L<sup>−1</sup> of O<sub>3</sub> for 720 min) on the quality and phytochemical content of broccoli florets during postharvest storage. The optimal dose was found at 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min, from the color retention of broccoli florets exposed to the gas treatment. Overall, the antioxidant capacity of the florets was significantly affected by both doses of O<sub>3</sub> compared to the non-exposed florets. The profile of glucosinolates was determined for up to 14 days in broccoli florets stored at 4 °C by LC-MS. The amount of total glucobrassicins and total hydroxy-cinnamates in florets significantly (<i>p</i> ≤ 0.05) improved by the application of 5 µL L<sup>−1</sup> of O<sub>3</sub> for 60 min compared to non-treated florets. The up-regulation of genes of the tryptophan-derived glucosinolate pathway was observed immediately after both treatments. The gene expression of CYP79A2 and CYP79B3 in broccoli was significantly higher in broccoli florets exposed to 5 µL L<sup>−1</sup> of O<sub>3</sub> for 720 min compared to non-exposed florets. Although enhancement of secondary metabolites can be achieved by the fumigation of broccoli florets with low doses of ozone, quality parameters, particularly weight loss, can be compromised.https://www.mdpi.com/2304-8158/11/15/2195broccoliO<sub>3</sub>oxidative stressglucosinolateshydroxy-cinnamates |
| spellingShingle | Arturo Duarte-Sierra Charles F. Forney Minty Thomas Paul Angers Joseph Arul Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone Foods broccoli O<sub>3</sub> oxidative stress glucosinolates hydroxy-cinnamates |
| title | Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone |
| title_full | Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone |
| title_fullStr | Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone |
| title_full_unstemmed | Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone |
| title_short | Phytochemical Enhancement in Broccoli Florets after Harvest by Controlled Doses of Ozone |
| title_sort | phytochemical enhancement in broccoli florets after harvest by controlled doses of ozone |
| topic | broccoli O<sub>3</sub> oxidative stress glucosinolates hydroxy-cinnamates |
| url | https://www.mdpi.com/2304-8158/11/15/2195 |
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