Sensitive and reproducible cell-free methylome quantification with synthetic spike-in controls

Sensitive and reproducible cell-free methylome quantification with synthetic spike-in controls

Summary: Cell-free methylated DNA immunoprecipitation sequencing (cfMeDIP-seq) identifies genomic regions with DNA methylation, using a protocol adapted to work with low-input DNA samples and with cell-free DNA (cfDNA). We developed a set of synthetic spike-in DNA controls for cfMeDIP-seq to provide...

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Bibliographic Details
Main Authors: Samantha L. Wilson, Shu Yi Shen, Lauren Harmon, Justin M. Burgener, Tim Triche, Jr., Scott V. Bratman, Daniel D. De Carvalho, Michael M. Hoffman
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Cell Reports: Methods
Subjects:
cell-free methylated DNA immunoprecipitation
cfMeDIP
spike-in controls
DNA methylation
cell-free DNA
cfDNA
Online Access:http://www.sciencedirect.com/science/article/pii/S266723752200176X

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http://www.sciencedirect.com/science/article/pii/S266723752200176X

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