5’UTR sequences influence protein levels in Escherichia coli by regulating translation initiation and mRNA stability

A set of 41 synthetic 5’UTRs with different theoretical translation initiation rates were generated to explore the role of 5’UTRs in the regulation of protein levels in Escherichia coli. The roles of the synthetic 5’UTRs in regulating the expression of different reporter genes were analyzed in vivo....

Full description

Bibliographic Details
Main Authors: Fan Chen, Muriel Cocaign-Bousquet, Laurence Girbal, Sébastien Nouaille
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-12-01
Series:Frontiers in Microbiology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2022.1088941/full