| Summary: | (DDC)2Zn, disulfiram (DS) metabolite, has shown promising anticancer effects in vitro but further investigations in vivo are limited by its poor water solubility. In this study, liposomes are assessed as a delivery system for (DDC)2Zn. Liposomes were prepared by the thin-film hydration method, followed by high-pressure homogenisation (HPH) for size reduction. The nano-liposomes were then characterised by size, polydispersity index (PDI), zeta potential (ZP), drug loading and encapsulation efficiencies(DLE% and EE%), and MTT cytotoxicity assay. The HSPC-based (PBS) liposomes showed a nano-range of sizes (< 200nm), good PDI (<0.5) but moderate EE%(<40%). However, (DDC)2Zn liposomal formulations showed enhanced cytotoxic activities toward colorectal cancer cells. Therefore, liposomal formulations of(DDC)2Zn with improved DLE% and EE% might have immense potential in cancer therapy.
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