In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil

In silico analysis of mismatches in RT-qPCR assays of 177 SARS-CoV-2 sequences from Brazil

Abstract INTRODUCTION: Quantitative reverse transcription polymerase chain reaction (RT-qPCR) can detect the severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2) in a highly specific manner. However, a decrease in the specificity of PCR assays for their targets may lead to false negative r...

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Bibliographic Details
Main Authors: Renan da Silva Santos, Raissa Souza Caminha Bret, Ana Cristina de Oliveira Monteiro Moreira, Adriana Rolim Campos, Angelo Roncalli Alves e Silva, Danielle Malta Lima, Kaio Cesar Simiano Tavares
Format: Article
Language:English
Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2020-11-01
Series:Revista da Sociedade Brasileira de Medicina Tropical
Subjects:
SARS-CoV-2
COVID-19
Polymerase chain reaction
Diagnosis
Mismatches
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100668&tlng=en

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100668&tlng=en

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