In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate
Pacific chub mackerel (Scomber japonicus) is an important fish throughout the world, especially in East Asian countries, including Korea, China, and Japan. Protein hydrolysates from marine sources are commonly used as nutritional supplements, functional ingredients, and flavor enhancers in the food,...
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2018-12-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/6181.pdf |
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| author | Khawaja Muhammad Imran Bashir Md. Mohibbullah Jeong Hyeon An Ji-Yeon Choi Yong-Ki Hong Jae Hak Sohn Jin-Soo Kim Jae-Suk Choi |
| author_facet | Khawaja Muhammad Imran Bashir Md. Mohibbullah Jeong Hyeon An Ji-Yeon Choi Yong-Ki Hong Jae Hak Sohn Jin-Soo Kim Jae-Suk Choi |
| author_sort | Khawaja Muhammad Imran Bashir |
| collection | DOAJ |
| description | Pacific chub mackerel (Scomber japonicus) is an important fish throughout the world, especially in East Asian countries, including Korea, China, and Japan. Protein hydrolysates from marine sources are commonly used as nutritional supplements, functional ingredients, and flavor enhancers in the food, beverage, and pharmaceutical industries. Antioxidants isolated from fish are relatively easy to prepare, are cost effective, and have no reported side effects. Hence, the present study aimed to investigate the in vivo antioxidant activities of mackerel muscle protein hydrolysate (MMPH) prepared using Protamex. The in vivo bioactivities of MMPH were investigated in alcoholic fatty liver mice (C57BL/6). Serum alanine aminotransferase and aspartate aminotransferase levels were comparable in test and control mice, whereas serum triglyceride and lipid peroxidation levels significantly (p < 0.05; p < 0.001) decreased after administration of MMPH (100–500 mg kg−1), especially at a concentration of 100 mg kg−1. A significant (p < 0.05) reduction in xanthine oxidase activity was observed in all groups treated with MMPH (100–500 mg kg−1), as compared with the control group. Significantly (p < 0.05) higher superoxide dismutase (SOD) activity/protein expression and regulated catalase (CAT) activity/protein expression levels were observed in groups administered MMPH (100–500 mg kg−1), especially at a concentration of 100 mg kg−1. These results show that the abundant amino acids of S. japonicus play an important role in the cytosol of the liver cells by directly participating in the expression of xanthine oxidase and the detoxifying SOD and CAT proteins, thereby enhancing antioxidant ability and ultimately, inhibiting lipid peroxidation. This study demonstrated that muscle protein hydrolysate from S. japonicus has strong antioxidant activities. |
| first_indexed | 2024-03-09T07:10:31Z |
| format | Article |
| id | doaj.art-8e57e13a62824319947186156bc32bc1 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:10:31Z |
| publishDate | 2018-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-8e57e13a62824319947186156bc32bc12023-12-03T09:07:42ZengPeerJ Inc.PeerJ2167-83592018-12-016e618110.7717/peerj.6181In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysateKhawaja Muhammad Imran Bashir0Md. Mohibbullah1Jeong Hyeon An2Ji-Yeon Choi3Yong-Ki Hong4Jae Hak Sohn5Jin-Soo Kim6Jae-Suk Choi7Seafood Research Center, IACF, Silla University, Busan, Republic of KoreaDepartment of Fisheries and Marine Bioscience, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalgonj, BangladeshSeafood Research Center, IACF, Silla University, Busan, Republic of KoreaSoutheast Medi-Chem Institute, Busan, Republic of KoreaDepartment of Biotechnology, College of Fisheries Sciences, Pukyong National University, Busan, Republic of KoreaSeafood Research Center, IACF, Silla University, Busan, Republic of KoreaDepartment of Seafood and Aquaculture Science, Gyeongsang National University, Tongyeong-si, Gyeongsangnam-do, Republic of KoreaSeafood Research Center, IACF, Silla University, Busan, Republic of KoreaPacific chub mackerel (Scomber japonicus) is an important fish throughout the world, especially in East Asian countries, including Korea, China, and Japan. Protein hydrolysates from marine sources are commonly used as nutritional supplements, functional ingredients, and flavor enhancers in the food, beverage, and pharmaceutical industries. Antioxidants isolated from fish are relatively easy to prepare, are cost effective, and have no reported side effects. Hence, the present study aimed to investigate the in vivo antioxidant activities of mackerel muscle protein hydrolysate (MMPH) prepared using Protamex. The in vivo bioactivities of MMPH were investigated in alcoholic fatty liver mice (C57BL/6). Serum alanine aminotransferase and aspartate aminotransferase levels were comparable in test and control mice, whereas serum triglyceride and lipid peroxidation levels significantly (p < 0.05; p < 0.001) decreased after administration of MMPH (100–500 mg kg−1), especially at a concentration of 100 mg kg−1. A significant (p < 0.05) reduction in xanthine oxidase activity was observed in all groups treated with MMPH (100–500 mg kg−1), as compared with the control group. Significantly (p < 0.05) higher superoxide dismutase (SOD) activity/protein expression and regulated catalase (CAT) activity/protein expression levels were observed in groups administered MMPH (100–500 mg kg−1), especially at a concentration of 100 mg kg−1. These results show that the abundant amino acids of S. japonicus play an important role in the cytosol of the liver cells by directly participating in the expression of xanthine oxidase and the detoxifying SOD and CAT proteins, thereby enhancing antioxidant ability and ultimately, inhibiting lipid peroxidation. This study demonstrated that muscle protein hydrolysate from S. japonicus has strong antioxidant activities.https://peerj.com/articles/6181.pdfSOD and CAT protein expressionIn vivo antioxidant activityProtamexEnzymatic hydrolysisProtein Hydrolysate |
| spellingShingle | Khawaja Muhammad Imran Bashir Md. Mohibbullah Jeong Hyeon An Ji-Yeon Choi Yong-Ki Hong Jae Hak Sohn Jin-Soo Kim Jae-Suk Choi In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate PeerJ SOD and CAT protein expression In vivo antioxidant activity Protamex Enzymatic hydrolysis Protein Hydrolysate |
| title | In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate |
| title_full | In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate |
| title_fullStr | In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate |
| title_full_unstemmed | In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate |
| title_short | In vivo antioxidant activity of mackerel (Scomber japonicus) muscle protein hydrolysate |
| title_sort | in vivo antioxidant activity of mackerel scomber japonicus muscle protein hydrolysate |
| topic | SOD and CAT protein expression In vivo antioxidant activity Protamex Enzymatic hydrolysis Protein Hydrolysate |
| url | https://peerj.com/articles/6181.pdf |
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