Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes
Introduction: Extracellular vesicles (EVs) released by mesenchymal stem cells (MSCs) are theorized to mediate cartilage regeneration through paracrine actions. It is hypothesized that EVs derived from synovial fluid MSCs (SF-MSCs) will promote anabolic gene expressions and decrease catabolic markers...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-12-01
|
| Series: | Journal of Cartilage & Joint Preservation |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667254523000525 |
| _version_ | 1827590993152573440 |
|---|---|
| author | Haixiang Liang Dan Li Eric V. Neufeld Michael J. Sayegh Adam Kiridly Pablo Palacios Henintsoa Fanjaniaina Andriamifidy Pooja Swami Kenneth R. Zaslav Nicholas A. Sgaglione Daniel A. Grande |
| author_facet | Haixiang Liang Dan Li Eric V. Neufeld Michael J. Sayegh Adam Kiridly Pablo Palacios Henintsoa Fanjaniaina Andriamifidy Pooja Swami Kenneth R. Zaslav Nicholas A. Sgaglione Daniel A. Grande |
| author_sort | Haixiang Liang |
| collection | DOAJ |
| description | Introduction: Extracellular vesicles (EVs) released by mesenchymal stem cells (MSCs) are theorized to mediate cartilage regeneration through paracrine actions. It is hypothesized that EVs derived from synovial fluid MSCs (SF-MSCs) will promote anabolic gene expressions and decrease catabolic markers of in vitro osteoarthritic chondrocytes. These EVs will also demonstrate increased cartilage regeneration as measured in a rat osteoarthritis (OA) model. Objectives: This study tested the in-vitro anti-inflammation effects of using EVs from SF-MSCs on chondrocytes and in vivo study on chondroprotective effects of EVs with OA environment. Methods: In vitro, rat chondrocytes were exposed to EVs collected from SF-MSC that had been either exposed or unexposed to interleukin-1β (IL-1β). Gene expressions for various anabolic and catabolic markers were measured. In vivo, rats who received anterior cruciate ligament transection as an OA model with an osteoarthritic knee joint were exposed to weekly injections of SF-MSC-derived EVs at 2 different doses. The results were evaluated with histological study and scored using the Osteoarthritis Research Society International system. Serum cytokines were analyzed via enzyme-linked immunosorbent assay. Results: In vitro, chondrocytes exposed to SF-MSCs derived EVs demonstrated increased anabolic markers and decreased catabolic markers also with the EVs collected from interleukin-1β treated SF-MSCs. In vivo, rats that received EVs displayed lower Osteoarthritis Research Society International scores, more collagen type II alpha 1, and less matrix metalloproteinase-13 than the control groups. This anti-inflammation potential was systemic, as demonstrated by the decrease of serum proinflammatory cytokines. Conclusions: SF-MSCs-derived EVs were effective at promoting the expression of anabolic markers while simultaneously attenuating the production of proinflammatory markers in vitro and in vivo. These anti-inflammation effects were dose-dependent in vivo. The injection of EVs in the knee joint changed the cytokines in the serum. |
| first_indexed | 2024-03-09T01:25:30Z |
| format | Article |
| id | doaj.art-8eb093d275b8496dbe750825ac3194fb |
| institution | Directory Open Access Journal |
| issn | 2667-2545 |
| language | English |
| last_indexed | 2024-03-09T01:25:30Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Cartilage & Joint Preservation |
| spelling | doaj.art-8eb093d275b8496dbe750825ac3194fb2023-12-10T06:18:56ZengElsevierJournal of Cartilage & Joint Preservation2667-25452023-12-0134100146Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytesHaixiang Liang0Dan Li1Eric V. Neufeld2Michael J. Sayegh3Adam Kiridly4Pablo Palacios5Henintsoa Fanjaniaina Andriamifidy6Pooja Swami7Kenneth R. Zaslav8Nicholas A. Sgaglione9Daniel A. Grande10Orthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USAOrthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USADepartment of Orthopaedic Surgery, Northwell Health, Long Island Jewish Medical Center/North Shore University Hospital, Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USADepartment of Orthopaedic Surgery, Northwell Health, Long Island Jewish Medical Center/North Shore University Hospital, Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USADonald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USAOrthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USAOrthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USAOrthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA; Lenox Hill Hospital, Northwell Health, New York, NY, USAOrthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA; Department of Orthopaedic Surgery, Northwell Health, Long Island Jewish Medical Center/North Shore University Hospital, Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USAOrthopaedic Research Laboratory, Feinstein Institutes for Medical Research, Northwell Health, Manhasset, NY, USA; Department of Orthopaedic Surgery, Northwell Health, Long Island Jewish Medical Center/North Shore University Hospital, Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA; Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Hempstead, NY, USA; Daniel A. Grande, Department of Orthopaedic Surgery, Feinstein Institutes for Medical Research at Northwell Health, 350 Community Dr, Manhasset, NY 11030.Introduction: Extracellular vesicles (EVs) released by mesenchymal stem cells (MSCs) are theorized to mediate cartilage regeneration through paracrine actions. It is hypothesized that EVs derived from synovial fluid MSCs (SF-MSCs) will promote anabolic gene expressions and decrease catabolic markers of in vitro osteoarthritic chondrocytes. These EVs will also demonstrate increased cartilage regeneration as measured in a rat osteoarthritis (OA) model. Objectives: This study tested the in-vitro anti-inflammation effects of using EVs from SF-MSCs on chondrocytes and in vivo study on chondroprotective effects of EVs with OA environment. Methods: In vitro, rat chondrocytes were exposed to EVs collected from SF-MSC that had been either exposed or unexposed to interleukin-1β (IL-1β). Gene expressions for various anabolic and catabolic markers were measured. In vivo, rats who received anterior cruciate ligament transection as an OA model with an osteoarthritic knee joint were exposed to weekly injections of SF-MSC-derived EVs at 2 different doses. The results were evaluated with histological study and scored using the Osteoarthritis Research Society International system. Serum cytokines were analyzed via enzyme-linked immunosorbent assay. Results: In vitro, chondrocytes exposed to SF-MSCs derived EVs demonstrated increased anabolic markers and decreased catabolic markers also with the EVs collected from interleukin-1β treated SF-MSCs. In vivo, rats that received EVs displayed lower Osteoarthritis Research Society International scores, more collagen type II alpha 1, and less matrix metalloproteinase-13 than the control groups. This anti-inflammation potential was systemic, as demonstrated by the decrease of serum proinflammatory cytokines. Conclusions: SF-MSCs-derived EVs were effective at promoting the expression of anabolic markers while simultaneously attenuating the production of proinflammatory markers in vitro and in vivo. These anti-inflammation effects were dose-dependent in vivo. The injection of EVs in the knee joint changed the cytokines in the serum.http://www.sciencedirect.com/science/article/pii/S2667254523000525CartilageExtracellular vesiclesOsteoarthritisStem cellsSynovial fluid |
| spellingShingle | Haixiang Liang Dan Li Eric V. Neufeld Michael J. Sayegh Adam Kiridly Pablo Palacios Henintsoa Fanjaniaina Andriamifidy Pooja Swami Kenneth R. Zaslav Nicholas A. Sgaglione Daniel A. Grande Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes Journal of Cartilage & Joint Preservation Cartilage Extracellular vesicles Osteoarthritis Stem cells Synovial fluid |
| title | Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes |
| title_full | Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes |
| title_fullStr | Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes |
| title_full_unstemmed | Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes |
| title_short | Extracellular vesicles from synovial fluid-derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes |
| title_sort | extracellular vesicles from synovial fluid derived mesenchymal stem cells confer chondroprotective effects on in vitro and in vivo osteoarthritic chondrocytes |
| topic | Cartilage Extracellular vesicles Osteoarthritis Stem cells Synovial fluid |
| url | http://www.sciencedirect.com/science/article/pii/S2667254523000525 |
| work_keys_str_mv | AT haixiangliang extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT danli extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT ericvneufeld extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT michaeljsayegh extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT adamkiridly extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT pablopalacios extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT henintsoafanjaniainaandriamifidy extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT poojaswami extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT kennethrzaslav extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT nicholasasgaglione extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes AT danielagrande extracellularvesiclesfromsynovialfluidderivedmesenchymalstemcellsconferchondroprotectiveeffectsoninvitroandinvivoosteoarthriticchondrocytes |