Expression, Purification and Refolding of a Human Na<sub>V</sub>1.7 Voltage Sensing Domain with Native-like Toxin Binding Properties

Expression, Purification and Refolding of a Human Na<sub>V</sub>1.7 Voltage Sensing Domain with Native-like Toxin Binding Properties

The voltage-gated sodium channel Na<sub>V</sub>1.7 is an important target for drug development due to its role in pain perception. Recombinant expression of full-length channels and their use for biophysical characterization of interactions with potential drug candidates is challenging d...

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Bibliographic Details
Main Authors: Ryan V. Schroder, Leah S. Cohen, Ping Wang, Joekeem D. Arizala, Sébastien F. Poget
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Toxins
Subjects:
membrane protein refolding
voltage-gated sodium channel
voltage sensor
bacterial expression of mammalian proteins
peptide toxin
lipid reconstitution
Online Access:https://www.mdpi.com/2072-6651/13/10/722

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https://www.mdpi.com/2072-6651/13/10/722

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