Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

Time- and cost-effective production of untagged recombinant MVA by flow virometry and direct virus sorting

Abstract Background Recombinant MVAs (rMVAs) are widely used both in basic and clinical research. Our previously developed Red-to-Green Gene Swapping Method (RGGSM), a cytometry-based Cell-Sorting protocol, revolves around the transient expression of a green fluorescent cytoplasmic marker, to subseq...

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Bibliographic Details
Main Authors: Daniela Boselli, Maddalena Panigada, Simona Di Terlizzi, Monica Romanò, Emanuele Canonico, Chiara Villa, Claudia Minici, Eelco van Anken, Elisa Soprana, Antonio G. Siccardi
Format: Article
Language:English
Published: BMC 2023-07-01
Series:Journal of Translational Medicine
Subjects:
Recombinant MVA
rMVA
Flow Virometry
Virus-Sorting
EEV and IMV virions
Hemagglutinin/egfp fusion protein
Online Access:https://doi.org/10.1186/s12967-023-04353-7

Internet

https://doi.org/10.1186/s12967-023-04353-7

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