Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines
Curcumin, the primary bioactive component isolated from turmeric, has been found to possess a variety of biological functions, including anti-leukemia activity. However, the effect of curcumin in different leukemia cells vary. In this study, we demonstrated that curcumin induced the expression of AI...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Cancer Biology & Therapy |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15384047.2022.2058862 |
| _version_ | 1797404056745934848 |
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| author | Yuru Zhou Yunyuan Kong Mei Jiang Linju Kuang Jinhua Wan Shuyuan Liu Qian Zhang Kuai Yu Na Li Aiping Le Zhanglin Zhang |
| author_facet | Yuru Zhou Yunyuan Kong Mei Jiang Linju Kuang Jinhua Wan Shuyuan Liu Qian Zhang Kuai Yu Na Li Aiping Le Zhanglin Zhang |
| author_sort | Yuru Zhou |
| collection | DOAJ |
| description | Curcumin, the primary bioactive component isolated from turmeric, has been found to possess a variety of biological functions, including anti-leukemia activity. However, the effect of curcumin in different leukemia cells vary. In this study, we demonstrated that curcumin induced the expression of AIM2, IFI16, and NLRC4 inflammasomes in leukemia cells U937 by increasing the expression levels of ISG3 transcription factor complex, which activated caspase 1, promoted cleavage of GSDMD, and induced pyroptosis. We also found that pyroptosis executor GSDMD was not expressed in two curcumin-insensitive cells HL60 and K562 cells. In addition, exogenous overexpression of GSDMD by lentiviral transduction in K562 cells increased the anti-cancer activity of curcumin, and inhibiting the expression of GSDMD by shRNA enhanced U937 cells to resist curcumin. The results showed that inducing pyroptosis is a novel mechanism underlying the anti-leukemia effects of curcumin. |
| first_indexed | 2024-03-09T02:47:35Z |
| format | Article |
| id | doaj.art-8ee116e1706c410fb5ddaa3d0ebcff76 |
| institution | Directory Open Access Journal |
| issn | 1538-4047 1555-8576 |
| language | English |
| last_indexed | 2024-03-09T02:47:35Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj.art-8ee116e1706c410fb5ddaa3d0ebcff762023-12-05T15:58:13ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762022-12-0123132833510.1080/15384047.2022.20588622058862Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell linesYuru Zhou0Yunyuan Kong1Mei Jiang2Linju Kuang3Jinhua Wan4Shuyuan Liu5Qian Zhang6Kuai Yu7Na Li8Aiping Le9Zhanglin Zhang10The First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityThe First Affiliated Hospital of Nanchang UniversityCurcumin, the primary bioactive component isolated from turmeric, has been found to possess a variety of biological functions, including anti-leukemia activity. However, the effect of curcumin in different leukemia cells vary. In this study, we demonstrated that curcumin induced the expression of AIM2, IFI16, and NLRC4 inflammasomes in leukemia cells U937 by increasing the expression levels of ISG3 transcription factor complex, which activated caspase 1, promoted cleavage of GSDMD, and induced pyroptosis. We also found that pyroptosis executor GSDMD was not expressed in two curcumin-insensitive cells HL60 and K562 cells. In addition, exogenous overexpression of GSDMD by lentiviral transduction in K562 cells increased the anti-cancer activity of curcumin, and inhibiting the expression of GSDMD by shRNA enhanced U937 cells to resist curcumin. The results showed that inducing pyroptosis is a novel mechanism underlying the anti-leukemia effects of curcumin.http://dx.doi.org/10.1080/15384047.2022.2058862curcumininflammasomepyroptosisleukemiagsdmd |
| spellingShingle | Yuru Zhou Yunyuan Kong Mei Jiang Linju Kuang Jinhua Wan Shuyuan Liu Qian Zhang Kuai Yu Na Li Aiping Le Zhanglin Zhang Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines Cancer Biology & Therapy curcumin inflammasome pyroptosis leukemia gsdmd |
| title | Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines |
| title_full | Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines |
| title_fullStr | Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines |
| title_full_unstemmed | Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines |
| title_short | Curcumin activates NLRC4, AIM2, and IFI16 inflammasomes and induces pyroptosis by up-regulated ISG3 transcript factor in acute myeloid leukemia cell lines |
| title_sort | curcumin activates nlrc4 aim2 and ifi16 inflammasomes and induces pyroptosis by up regulated isg3 transcript factor in acute myeloid leukemia cell lines |
| topic | curcumin inflammasome pyroptosis leukemia gsdmd |
| url | http://dx.doi.org/10.1080/15384047.2022.2058862 |
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