Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration

Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration

The ability to regulate gene activity spatially and temporally is essential to investigate cell-type-specific gene function during development and in postembryonic processes and disease models. The Cre/lox system has been widely used for performing cell and tissue-specific conditional analysis of ge...

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Bibliographic Details
Main Authors: Fang Liu, Sekhar Kambakam, Maira P Almeida, Zhitao Ming, Jordan M Welker, Wesley A Wierson, Laura E Schultz-Rogers, Stephen C Ekker, Karl J Clark, Jeffrey J Essner, Maura McGrail
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2022-06-01
Series:eLife
Subjects:
zebrafish
Cre/lox
conditional rescue and inactivation
CRISPR-Cas9
precision targeted integration
GeneWeld
Online Access:https://elifesciences.org/articles/71478

Internet

https://elifesciences.org/articles/71478

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