Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration
The ability to regulate gene activity spatially and temporally is essential to investigate cell-type-specific gene function during development and in postembryonic processes and disease models. The Cre/lox system has been widely used for performing cell and tissue-specific conditional analysis of ge...
Main Authors: | Fang Liu, Sekhar Kambakam, Maira P Almeida, Zhitao Ming, Jordan M Welker, Wesley A Wierson, Laura E Schultz-Rogers, Stephen C Ekker, Karl J Clark, Jeffrey J Essner, Maura McGrail |
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Format: | Article |
Language: | English |
Published: |
eLife Sciences Publications Ltd
2022-06-01
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Series: | eLife |
Subjects: | |
Online Access: | https://elifesciences.org/articles/71478 |
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