Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway
The present study investigated the effect of Stevia residue extract (SRE) against oxidative stress in d-galactose (d-gal) induced aging mice. LC-MS/MS analysis revealed that SRE is a good source of chlorogenic acids. Biochemical results showed that SRE significantly increased the activities of super...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464618306200 |
| _version_ | 1818920068994564096 |
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| author | Lei Zhao Huaqing Yang Meili Xu Xin Wang Chengtao Wang Yunhe Lian Arshad Mehmood Haochen Dai |
| author_facet | Lei Zhao Huaqing Yang Meili Xu Xin Wang Chengtao Wang Yunhe Lian Arshad Mehmood Haochen Dai |
| author_sort | Lei Zhao |
| collection | DOAJ |
| description | The present study investigated the effect of Stevia residue extract (SRE) against oxidative stress in d-galactose (d-gal) induced aging mice. LC-MS/MS analysis revealed that SRE is a good source of chlorogenic acids. Biochemical results showed that SRE significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total antioxidant capacity (T-AOC), and decreased acetylcholinesterase (AChE) activity and malondialdehyde (MDA) level in serum, liver or brain of d-gal induced aging mice. At 200 mg/kg, SRE up-regulated the mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream target genes including GPx1, CAT, SOD1, quinone oxidoreductase-1 (NQO1) and heme oxygenase-1 (HO-1) in d-gal treated mice liver. SRE also up-regulated the protein expression of Nrf2, HO-1 and the ratio of phosphorylated Akt to Akt (pAkt/Akt) in d-gal treated mice liver. These findings suggest that SRE is able to protect against oxidative stress in d-gal induced aging model via activation of Akt/Nrf2/HO-1 pathway. In conclusion, SRE may provide a promising dietary approach for the prevention or alleviation of oxidative stress and age-related conditions. |
| first_indexed | 2024-12-20T01:15:53Z |
| format | Article |
| id | doaj.art-8f11fd8a93fb4cbcbea88efc05a715ed |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T01:15:53Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-8f11fd8a93fb4cbcbea88efc05a715ed2022-12-21T19:58:34ZengElsevierJournal of Functional Foods1756-46462019-01-0152587595Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathwayLei Zhao0Huaqing Yang1Meili Xu2Xin Wang3Chengtao Wang4Yunhe Lian5Arshad Mehmood6Haochen Dai7Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaChenguang Biotech Group Co., Ltd., Handan 057250, Hebei, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaBeijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, China; Corresponding authors at: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China (C. Wang).Chenguang Biotech Group Co., Ltd., Handan 057250, Hebei, China; Corresponding authors at: Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China (C. Wang).Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing 100048, China; Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University, Beijing 100048, ChinaDepartment of Food Science, University of Massachusetts, Amherst, MA 01003, USAThe present study investigated the effect of Stevia residue extract (SRE) against oxidative stress in d-galactose (d-gal) induced aging mice. LC-MS/MS analysis revealed that SRE is a good source of chlorogenic acids. Biochemical results showed that SRE significantly increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and total antioxidant capacity (T-AOC), and decreased acetylcholinesterase (AChE) activity and malondialdehyde (MDA) level in serum, liver or brain of d-gal induced aging mice. At 200 mg/kg, SRE up-regulated the mRNA expression of nuclear factor erythroid 2-related factor 2 (Nrf2) and its downstream target genes including GPx1, CAT, SOD1, quinone oxidoreductase-1 (NQO1) and heme oxygenase-1 (HO-1) in d-gal treated mice liver. SRE also up-regulated the protein expression of Nrf2, HO-1 and the ratio of phosphorylated Akt to Akt (pAkt/Akt) in d-gal treated mice liver. These findings suggest that SRE is able to protect against oxidative stress in d-gal induced aging model via activation of Akt/Nrf2/HO-1 pathway. In conclusion, SRE may provide a promising dietary approach for the prevention or alleviation of oxidative stress and age-related conditions.http://www.sciencedirect.com/science/article/pii/S1756464618306200SteviaChlorogenic acidsHydroxycinnamate derivativesd-galactoseAgingOxidative stress |
| spellingShingle | Lei Zhao Huaqing Yang Meili Xu Xin Wang Chengtao Wang Yunhe Lian Arshad Mehmood Haochen Dai Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway Journal of Functional Foods Stevia Chlorogenic acids Hydroxycinnamate derivatives d-galactose Aging Oxidative stress |
| title | Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway |
| title_full | Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway |
| title_fullStr | Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway |
| title_full_unstemmed | Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway |
| title_short | Stevia residue extract ameliorates oxidative stress in d-galactose-induced aging mice via Akt/Nrf2/HO-1 pathway |
| title_sort | stevia residue extract ameliorates oxidative stress in d galactose induced aging mice via akt nrf2 ho 1 pathway |
| topic | Stevia Chlorogenic acids Hydroxycinnamate derivatives d-galactose Aging Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S1756464618306200 |
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