Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel
Abstract Reconstruction of severe osteochondral defects in articular cartilage and subchondral trabecular bone remains a challenging problem. The well-integrated bilayer osteochondral graft design expects to be guided the chondrogenic and osteogenic differentiation for stem cells and provides a prom...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-11-01
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| Series: | Journal of Biological Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13036-023-00389-x |
| _version_ | 1797414874083491840 |
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| author | Chih-Yun Lee Swathi Nedunchezian Sung-Yen Lin Yu-Feng Su Che-Wei Wu Shun-Cheng Wu Chung-Hwan Chen Chih-Kuang Wang |
| author_facet | Chih-Yun Lee Swathi Nedunchezian Sung-Yen Lin Yu-Feng Su Che-Wei Wu Shun-Cheng Wu Chung-Hwan Chen Chih-Kuang Wang |
| author_sort | Chih-Yun Lee |
| collection | DOAJ |
| description | Abstract Reconstruction of severe osteochondral defects in articular cartilage and subchondral trabecular bone remains a challenging problem. The well-integrated bilayer osteochondral graft design expects to be guided the chondrogenic and osteogenic differentiation for stem cells and provides a promising solution for osteochondral tissue repair in this study. The subchondral bone scaffold approach is based on the developed finer and denser 3D β-tricalcium phosphate (β-TCP) bioceramic scaffold process, which is made using a digital light processing (DLP) technology and the novel photocurable negative thermo-responsive (NTR) bioceramic slurry. Then, the concave-top disc sintered 3D-printed bioceramic incorporates the human adipose-derived stem cells (hADSCs) laden photo-cured hybrid biohydrogel (HG + 0.5AFnSi) comprised of hyaluronic acid methacryloyl (HAMA), gelatin methacryloyl (GelMA), and 0.5% (w/v) acrylate-functionalized nano-silica (AFnSi) crosslinker. The 3D β-TCP bioceramic compartment is used to provide essential mechanical support for cartilage regeneration in the long term and slow biodegradation. However, the apparent density and compressive strength of the 3D β-TCP bioceramics can be obtained for ~ 94.8% theoretical density and 11.38 ± 1.72 MPa, respectively. In addition, the in vivo results demonstrated that the hADSC + HG + 0.5AFnSi/3D β-TCP of the bilayer osteochondral graft showed a much better osteochondral defect repair outcome in a rabbit model. The other word, the subchondral bone scaffold of 3D β-TCP bioceramic could accelerate the bone formation and integration with the adjacent host cancellous tissue at 12 weeks after surgery. And then, a thicker cartilage layer with a smooth surface and uniformly aligned chondrocytes were observed by providing enough steady mechanical support of the 3D β-TCP bioceramic scaffold. |
| first_indexed | 2024-03-09T05:40:20Z |
| format | Article |
| id | doaj.art-8f236a052982405bb33c13be70a382b0 |
| institution | Directory Open Access Journal |
| issn | 1754-1611 |
| language | English |
| last_indexed | 2024-03-09T05:40:20Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj.art-8f236a052982405bb33c13be70a382b02023-12-03T12:25:37ZengBMCJournal of Biological Engineering1754-16112023-11-0117112410.1186/s13036-023-00389-xBilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogelChih-Yun Lee0Swathi Nedunchezian1Sung-Yen Lin2Yu-Feng Su3Che-Wei Wu4Shun-Cheng Wu5Chung-Hwan Chen6Chih-Kuang Wang7Ph.D. Program in Life Sciences, College of Life Science, Kaohsiung Medical UniversityRegenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical UniversityRegenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical UniversityFaculty of Post-Baccalaureate Medicine, College of Medicine, Kaohsiung Medical UniversityRegenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical UniversityRegenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical UniversityRegenerative Medicine and Cell Therapy Research Center, Kaohsiung Medical UniversityPh.D. Program in Life Sciences, College of Life Science, Kaohsiung Medical UniversityAbstract Reconstruction of severe osteochondral defects in articular cartilage and subchondral trabecular bone remains a challenging problem. The well-integrated bilayer osteochondral graft design expects to be guided the chondrogenic and osteogenic differentiation for stem cells and provides a promising solution for osteochondral tissue repair in this study. The subchondral bone scaffold approach is based on the developed finer and denser 3D β-tricalcium phosphate (β-TCP) bioceramic scaffold process, which is made using a digital light processing (DLP) technology and the novel photocurable negative thermo-responsive (NTR) bioceramic slurry. Then, the concave-top disc sintered 3D-printed bioceramic incorporates the human adipose-derived stem cells (hADSCs) laden photo-cured hybrid biohydrogel (HG + 0.5AFnSi) comprised of hyaluronic acid methacryloyl (HAMA), gelatin methacryloyl (GelMA), and 0.5% (w/v) acrylate-functionalized nano-silica (AFnSi) crosslinker. The 3D β-TCP bioceramic compartment is used to provide essential mechanical support for cartilage regeneration in the long term and slow biodegradation. However, the apparent density and compressive strength of the 3D β-TCP bioceramics can be obtained for ~ 94.8% theoretical density and 11.38 ± 1.72 MPa, respectively. In addition, the in vivo results demonstrated that the hADSC + HG + 0.5AFnSi/3D β-TCP of the bilayer osteochondral graft showed a much better osteochondral defect repair outcome in a rabbit model. The other word, the subchondral bone scaffold of 3D β-TCP bioceramic could accelerate the bone formation and integration with the adjacent host cancellous tissue at 12 weeks after surgery. And then, a thicker cartilage layer with a smooth surface and uniformly aligned chondrocytes were observed by providing enough steady mechanical support of the 3D β-TCP bioceramic scaffold.https://doi.org/10.1186/s13036-023-00389-xOsteochondralTissue engineeringBioceramic scaffoldDigital light processingHyaluronic acid methacryloylGelatin methacryloyl |
| spellingShingle | Chih-Yun Lee Swathi Nedunchezian Sung-Yen Lin Yu-Feng Su Che-Wei Wu Shun-Cheng Wu Chung-Hwan Chen Chih-Kuang Wang Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel Journal of Biological Engineering Osteochondral Tissue engineering Bioceramic scaffold Digital light processing Hyaluronic acid methacryloyl Gelatin methacryloyl |
| title | Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel |
| title_full | Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel |
| title_fullStr | Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel |
| title_full_unstemmed | Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel |
| title_short | Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel |
| title_sort | bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3d printed bioceramic and human adipose derived stem cells laden biohydrogel |
| topic | Osteochondral Tissue engineering Bioceramic scaffold Digital light processing Hyaluronic acid methacryloyl Gelatin methacryloyl |
| url | https://doi.org/10.1186/s13036-023-00389-x |
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