Subculturing and Gram staining of blood cultures flagged negative by the BACTEC™ FX system: Optimizing the workflow for detection of Cryptococcus neoformans in clinical specimens

Subculturing and Gram staining of blood cultures flagged negative by the BACTEC™ FX system: Optimizing the workflow for detection of Cryptococcus neoformans in clinical specimens

ObjectiveTo investigate whether an incubation time of 5 days (Aerobic/F, Anaerobic/F) and 14 days (Myco/F) blood culture bottles is sufficient to prevent false-negative results.MethodsWe evaluated 1,244 blood bottles (344 patients) defined as negative by the BACTEC™ FX system. We also reviewed publi...

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Bibliographic Details
Main Authors: Lingli Liu, Lijun Du, Shuquan He, Tianshu Sun, Fanrong Kong, Yali Liu, Yingchun Xu
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Microbiology
Subjects:
bloodstream infection
subculture
blood culture
BACTEC™ FX
Cryptococcus neoformans
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2023.1113817/full

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https://www.frontiersin.org/articles/10.3389/fmicb.2023.1113817/full

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