Dramatic Improvement of CRISPR/Cas9 Editing in <named-content content-type="genus-species">Candida albicans</named-content> by Increased Single Guide RNA Expression

ABSTRACT The clustered regularly interspaced short palindromic repeat system with CRISPR-associated protein 9 nuclease (CRISPR/Cas9) has emerged as a versatile tool for genome editing in Candida albicans. Mounting evidence from other model systems suggests that the intracellular levels of single gui...

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Bibliographic Details
Main Authors: Henry Ng, Neta Dean
Format: Article
Language:English
Published: American Society for Microbiology 2017-04-01
Series:mSphere
Subjects:
Online Access:https://journals.asm.org/doi/10.1128/mSphere.00385-16