The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.)
Aiming to achieve a precise method with easy application, this study presents the development and validation of a fast method for quantification of vitamin DL-α-tocopherol in quinoa seeds. The methodology was based on an extraction procedure using ultrasonic bath and determination by normal-phase HP...
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| Format: | Article |
| Language: | English |
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Institute for Food Technology, Novi Sad
2014-01-01
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| Series: | Food and Feed Research |
| Subjects: | |
| Online Access: | http://scindeks-clanci.ceon.rs/data/pdf/2217-5369/2014/2217-53691402147P.pdf |
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| author | Popović Sanja J. Kostadinović Ljiljana M. Brkljača Jovana S. Krulj Jelena A. Manojlović Maja S. Solarov-Bodroža Marija I. |
| author_facet | Popović Sanja J. Kostadinović Ljiljana M. Brkljača Jovana S. Krulj Jelena A. Manojlović Maja S. Solarov-Bodroža Marija I. |
| author_sort | Popović Sanja J. |
| collection | DOAJ |
| description | Aiming to achieve a precise method with easy application, this study presents the development and validation of a fast method for quantification of vitamin DL-α-tocopherol in quinoa seeds. The methodology was based on an extraction procedure using ultrasonic bath and determination by normal-phase HPLC with UV-VIS detector. Validation parameters showed adequate linearity, relative standard deviations between 0.5 and 0.8% (n=10), limits of detection and quantification were 3.0 ng/mL and 11.0 ng/mL, respectively. Moreover, testing the robustness of the method suggested that it was not changeable with time or condition. The results showed that this method is accurate and simple and thus applicable in laboratories for determination of DL-α-tocopherol in quinoa seeds. |
| first_indexed | 2024-04-12T21:36:55Z |
| format | Article |
| id | doaj.art-8f517fc5b06a40bda376872b0f74cde2 |
| institution | Directory Open Access Journal |
| issn | 2217-5369 2217-5660 |
| language | English |
| last_indexed | 2024-04-12T21:36:55Z |
| publishDate | 2014-01-01 |
| publisher | Institute for Food Technology, Novi Sad |
| record_format | Article |
| series | Food and Feed Research |
| spelling | doaj.art-8f517fc5b06a40bda376872b0f74cde22022-12-22T03:15:52ZengInstitute for Food Technology, Novi SadFood and Feed Research2217-53692217-56602014-01-0141214715210.5937/FFR1402147P2217-53691402147PThe development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.)Popović Sanja J.0Kostadinović Ljiljana M.1Brkljača Jovana S.2Krulj Jelena A.3Manojlović Maja S.4Solarov-Bodroža Marija I.5Institute for Food Technology, Novi Sad, Novi Sad, SerbiaInstitute for Food Technology, Novi Sad, Novi Sad, SerbiaInstitute for Food Technology, Novi Sad, Novi Sad, SerbiaInstitute for Food Technology, Novi Sad, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Agriculture, Novi Sad, SerbiaInstitute for Food Technology, Novi Sad, Novi Sad, SerbiaAiming to achieve a precise method with easy application, this study presents the development and validation of a fast method for quantification of vitamin DL-α-tocopherol in quinoa seeds. The methodology was based on an extraction procedure using ultrasonic bath and determination by normal-phase HPLC with UV-VIS detector. Validation parameters showed adequate linearity, relative standard deviations between 0.5 and 0.8% (n=10), limits of detection and quantification were 3.0 ng/mL and 11.0 ng/mL, respectively. Moreover, testing the robustness of the method suggested that it was not changeable with time or condition. The results showed that this method is accurate and simple and thus applicable in laboratories for determination of DL-α-tocopherol in quinoa seeds.http://scindeks-clanci.ceon.rs/data/pdf/2217-5369/2014/2217-53691402147P.pdfDL-α-tocopherolquinoa seedHPLC |
| spellingShingle | Popović Sanja J. Kostadinović Ljiljana M. Brkljača Jovana S. Krulj Jelena A. Manojlović Maja S. Solarov-Bodroža Marija I. The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.) Food and Feed Research DL-α-tocopherol quinoa seed HPLC |
| title | The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.) |
| title_full | The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.) |
| title_fullStr | The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.) |
| title_full_unstemmed | The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.) |
| title_short | The development and validation of HPLC method for quantification of DL-α-tocopherol in quinoa seeds (Chenopodium quinoa willd.) |
| title_sort | development and validation of hplc method for quantification of dl α tocopherol in quinoa seeds chenopodium quinoa willd |
| topic | DL-α-tocopherol quinoa seed HPLC |
| url | http://scindeks-clanci.ceon.rs/data/pdf/2217-5369/2014/2217-53691402147P.pdf |
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