RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a

RNA-Independent DNA Cleavage Activities of Cas9 and Cas12a

CRISPR-Cas systems provide bacteria and archaea with sequence-specific protection against invading mobile genetic elements. In the presence of divalent metal ions, Cas9 and Cas12a (formerly Cpf1) proteins target and cleave DNA that is complementary to a cognate guide RNA. The recognition of a protos...

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Bibliographic Details
Main Authors: Ramya Sundaresan, Hari Priya Parameshwaran, S.D. Yogesha, Mark Walter Keilbarth, Rakhi Rajan
Format: Article
Language:English
Published: Elsevier 2017-12-01
Series:Cell Reports
Subjects:
CRISPR
Cas9 endonucleases
Cpf1
Cas12a
Francisella tularensis novicida
RNA-independent DNA cleavage
Mn2+-specific CRISPR activity
SpyCas9
FnoCas9
FnoCas12a
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717317849

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http://www.sciencedirect.com/science/article/pii/S2211124717317849

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