Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

Systematic Identification of the Optimal Housekeeping Genes for Accurate Transcriptomic and Proteomic Profiling of Tissues following Complex Traumatic Injury

Trauma triggers critical molecular and cellular signaling cascades that drive biological outcomes and recovery. Variations in the gene expression of common endogenous reference housekeeping genes (HKGs) used in data normalization differ between tissue types and pathological states. Systematically, w...

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Bibliographic Details
Main Authors: Andrea H. Dragon, Cassie J. Rowe, Alisha M. Rhodes, Olivia L. Pak, Thomas A. Davis, Elsa Ronzier
Format: Article
Language:English
Published: MDPI AG 2023-02-01
Series:Methods and Protocols
Subjects:
trauma
gene expression
housekeeping gene (HKG)
reference gene normalization
gene expression stability
immunoblot
Online Access:https://www.mdpi.com/2409-9279/6/2/22

Internet

https://www.mdpi.com/2409-9279/6/2/22

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